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目的:研究左旋千金藤立定(SPD)激动内侧前额皮层(mPFC)D_1受体对皮层下伏隔核DA诱发释放的影响。方法:6-羟基多巴胺损伤大鼠mPFC两周后,同侧皮层内微量注射SPD,微透析检测电刺激中脑腹侧被盖区(VTA)或苯丙胺(AMP)诱导的NAc DA释放。结果:mPFC DA耗竭未改变NAc DA的基础水平和电刺激VTA诱发的DA释放,却明显易化AMP灌流诱发的NAc DA释放,表明mPFC DA系统参与调节NAc DA的诱发释放。mPFC内微量注射SPD未能改变电刺激VTA诱发的DA释放,但显著减弱AMP对NAc DA的诱发释放;该作用可被D_1拮抗剂Sch-23390部分翻转,而D_2拮抗剂spiperone无作用。结论:SPD强化mPFC D_1受体对皮层下伏隔核DA释放的抑制性调节作用。
Objective: To investigate the effect of D-limonene (SPD) agonist medial prefrontal cortex (D-1) receptor on DA-induced release of subcortical nucleus accumbens. Methods: SPD was injected into the ipsilateral cortex twice a week after exposure to 6-hydroxydopamine. The release of NAc DA induced by electrical stimulation of ventral tegmental area (VTA) or amphetamine (AMP) was detected by microdialysis. RESULTS: Depletion of mPFC DA did not alter basal levels of NAc DA and electrical stimulation of VTA-induced DA release, but significantly facilitated AMP perfusion-induced release of NAc DA, indicating that mPFC DA system is involved in regulating the release of NAc DA. Microinjection of SPD into mPFC did not change the release of DA induced by electrical stimulation of VTA, but significantly attenuated the release of NAc DA by AMP. This effect could be partially reversed by D-1 antagonist Sch-23390, but no effect was observed by D 2 antagonist spiperone. CONCLUSIONS: The inhibitory effect of SPD-enhanced mPFC D_1 receptor on DA release in subcortical nucleus accumbens.