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目的:参照欧洲药典、英国药典和美国药典,以大蒜辣素为指标成分对大蒜药材质量标准进行研究。方法:以水为介质,碾碎大蒜,蒜氨酸和蒜酶充分反应,采用硅胶G薄层板,以甲苯-乙酸乙酯(10∶3)为展开剂展开,薄层色谱鉴别大蒜药材中大蒜辣素成分;采用Hypersil BDS C18色谱柱(4.6 mm×250 mm,5μm),以羟苯丁酯内标为替代对照品,甲醇-1%甲酸(60∶40)为流动相,流速0.8 mL·min-1,柱温35℃,检测波长254 nm,高效液相色谱测定大蒜药材中潜在大蒜辣素含量。结果:大蒜药材供试品薄层色谱图中有一特征斑点与大蒜辣素对照品斑点一致;高效液相色谱法测得不同产地大蒜药材中潜在大蒜辣素含量(按无水物计算)在0.59%～1.05%范围内。结论:本研究以大蒜辣素为指标成分对大蒜药材进行薄层色谱鉴别和高效液相色谱含量测定,并以干品计算大蒜药材中潜在大蒜辣素含量,为修订2015年版中国药典中大蒜质量标准提供参考。
Objective: With reference to the European Pharmacopoeia, the British Pharmacopoeia and the United States Pharmacopoeia, allicin was used as an index to study the quality standard of garlic medicinal materials. Methods: With water as the medium, garlic, garlic and garlic enzyme were fully crushed. Silica gel G plate was used to develop with toluene-ethyl acetate (10: 3) The content of allicin was replaced by Hypersil BDS C18 column (4.6 mm × 250 mm, 5 μm) with the standard of n-butylpalate as the mobile phase. The mobile phase consisted of methanol-1% formic acid (60:40) Min-1, the column temperature was 35 ℃, detection wavelength was 254 nm, and the content of allicin in garlic was determined by HPLC. Results: Garlic medicinal materials for the test sample thin-layer chromatogram has a characteristic spots and allicin reference substance spot consistent; HPLC determination of garlic from different areas of potential allicin content (calculated by anhydrous) 0.59 % ~ 1.05% range. Conclusion: In this study, allicin was used as an index component to determine the content of garlic medicinal materials by TLC and HPLC, and the content of allicin in garlic medicinal materials was calculated by dry product. In order to revise the garlic quality of Chinese Pharmacopoeia Standard for reference.