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制备了纳米颗粒的银溶胶,研究了核黄素水溶液中加入纳米银后荧光光谱和吸收光谱的变化。纳米银可以使核黄素的荧光发生猝灭,它们之间的相互作用属于静态猝灭机制,求出了猝灭常数Ksv=1.04×104L/mol,Kq=1.5×1013L.mol-1.s-1。纳米银对核黄素荧光的这种静态猝灭引起吸收光谱发生变化,随着纳米银浓度的增大,吸收强度也不断增大,而且吸收峰从371 nm红移至376 nm、445 nm蓝移至441 nm。研究表明,核黄素结构中发色团上尿嘧啶端的氮杂原子及羰基中的氧原子与纳米银形成了复合物,核黄素与纳米银之间存在能量转移,从而导致了核黄素光谱的变化。
The silver nanoparticles of nanoparticles were prepared and the changes of fluorescence spectra and absorption spectra of the nanosized silver nanoparticles were studied. Nanosilver can quench the fluorescence of riboflavin. The interaction between them belongs to the static quenching mechanism. The quenching constants Ksv = 1.04 × 104L / mol, Kq = 1.5 × 1013L.mol-1.s -1. The static quenching of nanosilver to riboflavin fluorescence causes the absorption spectrum to change. With the increase of the concentration of nanosilver, the absorption intensity increases, and the absorption peak shifts from 371 nm to 376 nm, 445 nm blue Move to 441 nm. Studies have shown that the uranyl terminal nitrogen atom and the oxygen atom in the carbonyl group of the chromophore in the riboflavin structure form a complex with the nano-silver, and there is energy transfer between the riboflavin and the nano-silver, Spectral changes.