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采用胰酶消化法建立了大鼠黄体细胞体外分离方法。在整个操作过程中 ,黄体细胞存活率均在95 %以上。研究结果表明 :孕期 14～ 2 0天大鼠、黄体细胞密度 10 6个 / ml、培养时间 4小时是适宜的体外分离培养条件。在对某市自来水厂氯化饮用水有机浓集物 (每孔相当于 5 0 0、10 0 0、30 0 0、5 0 0 0 ml自来水 )进行毒性测试时发现 :各试验浓度组均可使黄体细胞孕酮分泌量显著增加 (P<0 .0 1) ,呈现出明显的剂量 -效应关系。故认为氯化饮用水有机浓集物可导致大鼠黄体细胞孕酮分泌量显著增加 ,从而影响黄体细胞的正常生理功能。
The method of in vitro isolation of rat luteal cells was established by trypsin digestion. Throughout the operation, luteal cell survival rates were above 95%. The results show that: 14 to 20 days pregnant pregnant rats, luteal cell density 106 / ml, culture time 4 hours is suitable for in vitro culture conditions. In a municipal water plant chlorinated drinking water organic concentrates (each hole is equivalent to 500, 100000, 30000, 50000ml tap water) toxicity test found that: the concentration of each test group Progesterone secretion of luteal cells increased significantly (P <0.01), showing a significant dose-response relationship. Therefore, the organic concentration of chlorinated drinking water can lead to significantly increased progesterone secretion of rat luteal cells, thereby affecting the normal physiological function of luteal cells.