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目的:探讨HDAC1的异常表达以及HPV16感染与宫颈癌发病的相关性。方法:用PCR技术及免疫组织化学(SP)方法检测110例慢性宫颈炎组织及宫颈上皮内瘤样变组织和宫颈鳞癌组织中HPV16感染以及HDAC1的表达。结果:在慢性宫颈炎组、CINⅠ组、CINⅡ组、CINⅢ组和宫颈癌组中HPV16的感染率分别为10.0%、36.4%、50.0%、66.7%、75.0%,组间差异有统计学意义(P<0.05);HDAC1阳性表达率分别为0、27.3%、41.7%、54.2%和70.0%,各组间差异有统计学意义(P<0.05)。HPV16感染组的HDAC1阳性表达率高于HPV16未感染组,差异有统计学意义(P<0.05)。结论:HPV16感染可能是通过提高组蛋白去乙酰化酶的水平抑制转录,在宫颈癌的发生、发展中起作用。
Objective: To investigate the abnormal expression of HDAC1 and the correlation between HPV16 infection and the incidence of cervical cancer. Methods: HPV16 infection and HDAC1 expression in 110 cases of chronic cervicitis, cervical intraepithelial neoplasia and cervical squamous cell carcinoma were detected by PCR and immunohistochemistry (SP). Results: In chronic cervicitis group, the infection rates of HPV16 in CINⅠgroup, CINⅡgroup, CINⅢgroup and cervical cancer group were 10.0%, 36.4%, 50.0%, 66.7% and 75.0%, respectively. The difference between the two groups was statistically significant P <0.05). The positive rate of HDAC1 expression was 0, 27.3%, 41.7%, 54.2% and 70.0%, respectively. There was significant difference between the groups (P <0.05). The positive rate of HDAC1 expression in HPV16 infection group was higher than that in HPV16 non-infected group, the difference was statistically significant (P <0.05). Conclusion: HPV16 infection may inhibit the transcription by increasing the level of histone deacetylase and play a role in the occurrence and development of cervical cancer.