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目的:研究TgN(p53mt-LMP1)/HT转基因小鼠鼻腔和鼻咽黏膜上皮细胞增殖与p16、c-jun基因表达的关系。方法:TgN(p53mt-LMP1)/HT小鼠和野生型C57BL/6J小鼠分别分为诱癌组和对照组,即TgN(p53mt-LMP1)/HT摘要目的:研究TgN(p53mt-LMP1)/HT转基因小鼠鼻腔和鼻咽黏膜上皮细胞增殖与p16、c-jun基因表达的关诱癌组(TI)、TgN(p53mt-LMP1)/HT对照组(TC)、C57BL/6J诱癌组(CI)和C57BL/6J对照组(CC)4组,H-E染色法观察各组小鼠鼻腔和鼻咽黏膜上皮细胞增殖特征,比较癌前病变率;免疫组织化学染色法检测各组小鼠鼻腔和鼻咽黏膜上皮组织中p16、c-jun基因的表达水平。结果:TI组小鼠鼻腔或鼻咽黏膜上皮细胞非典型性增生明显,TI、TC、CI和CC组小鼠鼻腔和/或鼻咽黏膜上皮癌前病变率分别为90%、10%、0和0。与TC、CI和CC组相比,TI组鼻腔和鼻咽上皮细胞p16基因表达水平显著降低(P<0.01)和c-jun基因表达水平显著增高(P<0.01);与CC组相比,TC组两基因的表达也具有同样的变化趋势(P<0.01)。结论:TgN(p53mt-LMP1)/HT小鼠鼻腔和鼻咽黏膜上皮细胞增殖活性增加,二亚硝基哌嗪可提高TgN(p53mt-LMP1)/HT小鼠鼻腔和鼻咽黏膜上皮细胞的增殖活性,而细胞增殖活性增加与p16基因表达水平下调和c-jun基因表达水平上调密切相关。
Objective: To investigate the relationship between the proliferation of nasal mucosa and nasopharyngeal epithelial cells and the expression of p16 and c-jun in TgN (p53mt-LMP1) / HT transgenic mice. Methods: TgN (p53mt-LMP1) / HT mice and wild-type C57BL / 6J mice were divided into cancer-inducing group and control group, namely, TgN (p53mt-LMP1) HT transgenic mice nasopharyngeal and nasopharyngeal mucosa epithelial cell proliferation and p16, c-jun gene expression in the induced cancer group (TI), TgN (p53mt-LMP1) / HT control group (TC), C57BL / 6J cancer group CI) and C57BL / 6J control group (CC). The proliferative characteristics of nasal and nasopharyngeal mucosal epithelial cells in each group were observed by HE staining and the precancerous lesions were compared. Immunohistochemical staining was used to detect the nasal cavity and Expression of p16 and c-jun genes in nasopharyngeal mucosa epithelium. Results: The atypical hyperplasia of nasal or nasopharyngeal mucosal epithelial cells in TI group was obvious. The rates of nasal and / or nasopharyngeal mucosal precancerous lesions in TI, TC, CI and CC groups were 90%, 10%, 0 And 0. Compared with TC, CI and CC groups, the expression of p16 gene in nasal and nasopharyngeal epithelial cells was significantly decreased (P <0.01) and the expression of c-jun gene was significantly increased in TI group (P <0.01) TC gene expression of two genes also have the same trend (P <0.01). CONCLUSION: The proliferative activity of nasal and nasopharyngeal mucosal epithelial cells in TgN (p53mt-LMP1) / HT mice increased, and dinitrosopiperazine increased the proliferation of nasal and nasopharyngeal mucosal epithelial cells in TgN (p53mt-LMP1) / HT mice Activity, while increased cell proliferation activity is closely related to the down-regulation of p16 gene expression and the up-regulation of c-jun gene expression.