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目的了解安徽省肠出血性大肠杆菌O157:H7在宿主动物中的分布及其毒力基因携带状况,为控制O157:H7感染提供依据。方法用免疫磁珠分离法(IMS)对安徽省3个监测点的宿主动物粪便标本进行0157:H7的分离培养,并用多重引物PCR方法(MPCR)检测分离菌株的毒力基因。结果采集宿主动物粪便标本5 560份,检出O157:H737株,阳性率为0.7%,其中牛的阳性率最高,为1.1%。106株O157:H7菌株经过毒力基因测定,65.1%的菌株携带SLT、hly基因。O157:H7感染性腹泻的发病与家禽家畜的带菌正相关(R=0.87,P=0.01)。结论安徽省宿主动物中能检出肠出血性大肠杆菌O157:H7,且菌株携带毒力基因。提示要加强对宿主动物进行O157:H7的流行病学调查和监测工作。
Objective To understand the distribution and virulence of enterohemorrhagic Escherichia coli O157: H7 in host animals in Anhui Province, and provide a basis for controlling the infection of O157: H7. Methods The host animal stool samples from three monitoring sites in Anhui Province were isolated and purified by immunomagnetic bead method (IMS). 0157: H7 was isolated and purified. The virulence genes of the isolates were tested by multiplex PCR (MPCR). Results A total of 5605 fecal samples were collected from domestic animals and O157: H737 was detected. The positive rate was 0.7%. The positive rate of cattle was 1.1%. The virulence genes of 106 O157: H7 strains were tested, and 65.1% strains carried SLT and hly genes. O157: The incidence of H7 infectious diarrhea was positively correlated with carriage of poultry to livestock (R = 0.87, P = 0.01). Conclusion Enterohemorrhagic Escherichia coli O157: H7 can be detected in the host animals of Anhui Province, and the strain carries virulence genes. Prompted to strengthen the host animal O157: H7 epidemiological survey and monitoring.