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目的 :研究免疫活性物质卡介苗基因组 DNA(BCG- DNA)对哮喘患者外周血单个核细胞 (PBMC) IFN- γ和 IL- 4产生的影响。 方法 :分离哮喘患者 PBMC,分别加入 BCG- DNA、地塞米松刺激培养 ,EL ISA检测培养上清中 IFN- γ及 IL- 4含量 ,RT- PCR检测 PBMC IFN-γ m RNA及 IL - 4 m RNA的表达量 ,并与空白对照组相比较。结果 :地塞米松在体外对哮喘患者PBMC产生的 IFN-γ m RNA和蛋白、IL - 4 m RNA和蛋白均有抑制作用 ;BCG- DNA抑制哮喘患者 PBMC IL - 4 m RNA和蛋白的分泌 ,而促进 IFN -γ m RNA和蛋白的分泌 ,与对照组比较差异显著 (P<0 .0 1 ) ,且在体外对 PBMC无毒性。 结论 :BCG-DNA不仅具有和地塞米松相同的下调 IL - 4表达的作用 ,而且还具有地塞米松所没有的诱导 IFN -γ表达的作用 ,利于纠正哮喘患者体内存在的 Th1 / Th2比例失衡状态 ,起到治疗作用
Objective: To study the effects of BCG-DNA on the production of IFN-γ and IL-4 in peripheral blood mononuclear cells (PBMCs) of asthmatic patients. Methods: The PBMC of asthmatic patients were isolated and cultured with BCG-DNA and dexamethasone respectively. The levels of IFN-γ and IL-4 in the culture supernatant were detected by ELISA. The levels of IFN-γmRNA and IL - 4m The amount of RNA expression was compared with the blank control group. Results: Dexamethasone inhibited IFN - γm RNA and protein, IL - 4m RNA and protein produced by PBMCs in asthma patients in vitro. BCG - DNA inhibited IL - 4 mRNA and protein secretion in PBMC of asthmatic patients, However, the secretion of IFN-γ m RNA and protein was significantly different from the control group (P <0.01), and non-toxic to PBMC in vitro. CONCLUSION: BCG-DNA not only has the same effect of down-regulating IL-4 as dexamethasone, but also induces the expression of IFN-γ which is not induced by dexamethasone, which is beneficial to correct imbalance of Th1 / Th2 ratio in asthma patients State, play a therapeutic role