论文部分内容阅读
目的观察清胰Ⅱ号颗粒剂对重症急性胰腺炎(SAP)肝损伤的保护作用。方法将24只SD大鼠随机平分为三组:假手术组(A组)、胰腺炎组(B组)、清胰Ⅱ号治疗组(C组)。A组仅开腹不制作SAP模型,B、C组以3%牛磺胆酸钠逆行注入大鼠胆胰管制作SAP模型,C组给予清胰Ⅱ号治疗,造模后于24 h取材。ELISA法检测血清白细胞介素(IL)-1、IL-6、IL-8,同时观察血淀粉酶、肝功能;余肝组织侵入10%福尔马林溶液以便病理检查。结果与A组相比,B组血清IL-1、IL-6、IL-8浓度异常升高(P<0.05);血清天门冬酸氨基转移酶(AST)、丙氨酸氨基转移酶(ALT)、乳酸脱氢酶(LDH)显著升高(P<0.01);C组与B组相比,同时血清IL-1、IL-6、IL-8浓度也异常下降(P<0.01),血清AST、ALT、LDH也显著降低(P<0.01);C组肝组织病理损害较B组减轻。结论清胰Ⅱ号颗粒剂可以减轻SAP肝损害,其机制可能通过抑制炎性介质释放。
Objective To observe the protective effect of Qingyi Ⅱ granule on hepatic injury in severe acute pancreatitis (SAP). Methods Twenty-four SD rats were randomly divided into three groups: sham-operation group (A group), pancreatitis group (B group) and Qing-qi Ⅱ treatment group (C group). Group A was only open abdomen without SAP model, Group B and C were treated with 3% sodium taurocholate retrograde injection into the biliary and pancreatic ducts of rats to make SAP model. Group C was treated with Qingyi No.2. Serum interleukin (IL) -1, IL-6 and IL-8 were detected by ELISA. Blood amylase and liver function were also observed. The remaining liver tissue was infiltrated with 10% formalin for pathological examination. Results Compared with group A, the concentrations of IL-1, IL-6 and IL-8 in group B were abnormally increased (P <0.05), the levels of serum aspartate aminotransferase (AST), alanine aminotransferase (P <0.01). Compared with group B, the concentrations of IL-1, IL-6 and IL-8 in serum of group C also decreased abnormally (P <0.01) AST, ALT and LDH also decreased significantly (P <0.01). The pathological changes of liver in group C were less than those in group B. Conclusion Qingyi Ⅱ granules can reduce SAP liver damage, the mechanism may be through inhibiting the release of inflammatory mediators.