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目的建立HPLC法同时测定抗感胶囊中绿原酸、咖啡酸、芍药苷、木犀草苷和芦丁的方法。方法采用DiamonsilTMC18色谱柱(250 mm×4.6 mm,5μm);柱温30℃;体积流量:1.0 mL/min;流动相:甲醇-0.5%冰醋酸,梯度洗脱;自动进样:20μL;检测波长:270 nm。结果在此色谱条件下5种成分可完全分离。绿原酸、咖啡酸、芍药苷、木犀草苷和芦丁的线性范围分别为0.231 0~3.465 0μg(r=1.000 0)、0.037 8~0.567 0μg(r=0.999 9)、0.292 4~4.386 0μg(r=0.999 7),0.045 2~0.678 0μg(r=0.999 8)、0.143 8~2.157μg(r=0.999 9)。平均回收率绿原酸为98.5%(RSD为1.1%),咖啡酸为98.4%(RSD为0.9%),芍药苷为97.3%(RSD为1.1%),木犀草苷为98.6%(RSD为1.0%),芦丁为99.9%(RSD为0.28%)。结论该方法专属性好、准确度高,可用于综合评价抗感胶囊的质量。
OBJECTIVE To establish a HPLC method for the simultaneous determination of chlorogenic acid, caffeic acid, paeoniflorin, luteolin and rutin in Kanggan capsule. Methods Diamonsil TMC18 column (250 mm × 4.6 mm, 5 μm) was used. The column temperature was 30 ℃. The volume flow rate was 1.0 mL / min. The mobile phase consisted of methanol-0.5% acetic acid and gradient elution. : 270 nm. Results under the chromatographic conditions of five components can be completely separated. The linear range of chlorogenic acid, caffeic acid, paeoniflorin, luteolin and rutin were 0.231 0 ~ 3.465 0μg (r = 1.000 0), 0.037 8 ~ 0.567 0μg (r = 0.999 9), 0.292 4 ~ 4.386 0μg (r = 0.999 7), 0.045 2 ~ 0.678 0μg (r = 0.999 8), 0.143 8 ~ 2.157μg (r = 0.999 9). The average recovery was 98.5% for chlorogenic acid (RSD 1.1%), caffeic acid 98.4% (RSD 0.9%), paeoniflorin 97.3% (RSD 1.1%) and luteolin as 98.6% (RSD 1.0 %), Rutin 99.9% (RSD 0.28%). Conclusion The method is of good specificity and high accuracy, and can be used to evaluate the quality of anti-capsule.