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探讨了人离体血经X线照射后,染色体培养过程中松胞素B(Cyt-B)及培养时间对分裂指数(MI)的影响。结果表明:①0～15Gy照射,培养体系中加入Cyt-B,MI均有所增加;10Gy以上影响较大,25Gy不再有影响。②6～25Gy,延长培养时间MI明显增加,在6～10Gy间仅靠延长时间即可获足够分析用的分裂像。③加Cyt-B的同时延长培养时间,其MI高于单纯加Cyt-B或延长时间者,特别是15Gy,照后只有加Cyt-B同时延长培养时间才能使MI增多至可供分析。以上结果提示:①辐射事故中当以染色体畸变估算大于6Gy受照者的剂量时,必须延长培养时间,有条件者加Cyt-B方可增加培养成功的机会,提供足够的分裂像。②改变培养条件,加Cyt-B并延长培养时间有可能制定出剂量上限高于5Gy的剂量效应刻度曲线,从而进一步提高其作为生物剂量计的临床应用价值。
To investigate the effect of cytochrome c (Cyt-B) and culture time on the mitotic index (MI) of human blood in vitro after X-ray irradiation. The results showed that: ①0 ~ 15Gy irradiation, Cyt-B, MI were increased in the culture system; 10Gy above the greater impact, 25Gy no longer affected. ② 6 ~ 25Gy, prolonged incubation time MI significantly increased, in the 6 ~ 10Gy only by extending the time to get enough for analysis of the split image. ③ add Cyt-B at the same time to extend the incubation time, the MI was higher than simply add Cyt-B or prolongation of time, especially 15Gy, according to only add Cyt-B while prolonging the incubation time to make MI increased for analysis. The above results suggest that: ① In the case of radiation accident, when the dose of more than 6 Gy dose is estimated by chromosomal aberration, the culture time must be prolonged. Cyt-B may be used to increase the chances of success of culture and provide sufficient mitotic images. (2) Changing the culture conditions, adding Cyt-B and prolonging the culture time, it is possible to formulate a dose-response calibration curve with a dose upper limit higher than 5 Gy to further improve its clinical application value as a biological dosimeter.