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用ｌｉｐｏｆｅｃｔｉｎ介导质粒ｐＬＸＳＮ和人神经营养素３（ｈＮＴ－３）的重组质粒ｐＬｈＮＴ－３ＳＮ转染ＰＡ３１７细胞，获得了较高滴度的重组逆转录病毒，感染成肌细胞后用ＰＣＲ和Ｎｏｒｔｈｅｒｎ杂交方法检测出ｎｅｏ基因与ｈＮＴ－３的ｍＲＮＡ。获得高表达工程细胞的关键在于培养基与培养温度的选择
PA317 cells were transfected with the recombinant plasmid pLhNT-3SN containing lipofectin plasmid pLXSN and human neurotrophic factor 3 (hNT-3), and the recombinant retrovirus with higher titer was obtained. After being infected into myoblasts, The neo gene and hNT-3 mRNA were detected. The key to obtaining highly engineered cells lies in the choice of medium and incubation temperature