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应用Centricon-10微浓缩器和组织培养方法,探讨备用根大鼠手术侧和非手术侧脊髓后角组织小于10kD和大于10kD两种分子量组分提取液对鸡胚背根节神经元的神经突起的促生长作用。结果显示:给予备用根大鼠手术侧脊髓后角组织大于10kD组分的提取液时,背根节神经元的神经突起密度较非手术例小于10kD组分、非手术侧大于10kD组分和手术侧小于10kD组分者密度明显增大.提示部分去传人纤维支配的脊髓后角组织大于10kD组分的提取液,可能含有某些促进培养鸡胚背根节神经元神经突起生长的神经营养物质.
Centricon-10 micro-concentrator and tissue culture methods were used to investigate the neurite outgrowth of chick embryo’s dorsal root ganglion neurons in the spare root and the non-surgical spinal dorsal horn of the spinal cord of less than 10kD and more than 10kD The role of promoting growth. The results showed that the neurite density of dorsal root ganglion neurons was less than 10kD in non-surgical cases and larger than 10kD in non-operative side and surgery Side of less than 10kD component density increased significantly. Prompt part of the descendants of fiber to the descending angle at the dorsal horn greater than 10kD component extract, may contain some to promote chick embryo nerve root neurite growth neurotrophic material.