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目的：研究血小板生成素（ＴＰＯ）及其他造血生长因子在体外对人血小板活化的影响。方法：应用荧光标记单克隆抗体和流式细胞技术。结果：终浓度 １２０ ｎｇ／ｍＬ的ＴＰＯ在体外能直接诱导人血小板活化，活化率为 ８．８９％～３９．９２％，中位数 １７．４３％，４０ｎｇ／ｍＬ的较低浓度 ＴＰＯ及 １００ｎｇ／ｍＬ粒细胞－巨噬细胞集落刺激因子（ＧＭ－ＣＳＦ）和白细胞介素－３（ＩＬ－３）对血小板活化无影响。结论：ＴＰＯ对血小板功能有促进作用，较高浓度的ＴＰＯ可直接诱导血小板活化。
Objective: To investigate the effect of thrombopoietin (TPO) and other hematopoietic growth factors on human platelet activation in vitro. Methods: Fluorescently labeled monoclonal antibodies and flow cytometry were used. Results: TPO with a final concentration of 120 ng / mL could directly induce human platelet activation in vitro with the activation rates of 8.89% -39.92%, median of 17.43%, lower concentrations of 40ng / mL TPO and 100ng / ML granulocyte-macrophage colony-stimulating factor (GM-CSF) and interleukin-3 (IL-3) had no effect on platelet activation. Conclusion: TPO can promote platelet function. Higher concentration of TPO can directly induce platelet activation.