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目的： 探讨端粒酶活化在喉癌和下咽癌发生发展中的作用。方法： 采用改良的银染端粒重复序列扩增法对５８ 例喉癌下咽癌组织、２５ 例癌旁组织和１０ 例癌前病变组织进行了端粒酶活性检测。结果： ５８ 例喉癌和下咽癌中５３ 例阳性， 阳性率９１４ ％ ； ２５ 例癌旁组织中３ 例阳性， 阳性率１２ ％ ； １０ 例癌前病变中７ 例阳性， 阳性率７０ ％ 。结论： 端粒酶活化在喉癌、下咽癌及其癌前病变组织中表达极高， 对阐明喉癌和下咽癌的发病机理和癌变风险估计有重要意义， 有可能成为咽喉癌早期诊断的生物学标记物。
Objective: To investigate the role of telomerase activation in the development of laryngeal carcinoma and hypopharyngeal carcinoma. Methods: Telomerase activity was detected in 58 cases of hypopharyngeal carcinoma of the laryngeal squamous cell carcinoma, 25 cases of paracancerous tissues and 10 cases of precancerous lesions using a modified silver-labeled telomere repeat amplification assay. Results: Of the 58 cases of laryngeal and hypopharyngeal carcinoma, 53 were positive and the positive rate was 914%. Three of 25 paracancerous tissues were positive and the positive rate was 12%. Seven of 10 precancerous lesions were positive and the positive rate was 70 %. Conclusion: The expression of telomerase activity in laryngeal carcinoma, hypopharyngeal carcinoma and its precancerous lesions is extremely high, which is of great significance in elucidating the pathogenesis of carcinogenesis of laryngeal carcinoma and hypopharyngeal carcinoma and the risk of cancer. It may be an early diagnosis of laryngeal cancer Of biological markers.