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目的观察牛磺酸对大鼠视网膜缺血再灌注损伤(retinal ischemia-reperfusion,RIR)的影响及其作用机制。方法将120只大鼠随机分为3组:对照组、缺血组、保护组,采用前房灌注液体形成14.63 kPa高眼压1 h的方法,建立RIR模型,连续3 d,2次/d,腹腔注射10%的牛磺酸,剂量为100 mg/kg,于手术前30 min加注1次。对照组给予同等剂量的生理盐水。两组缺血60 min后,分别再灌注0、2、6、12、24、48、72 h用比色法进行视网膜SOD、MDA、NO的测定;用光镜测量包埋切片的平均视网膜内层厚度(mean thickness of inner retinal layer,MTIRL)。结果牛磺酸能显著对抗大鼠视网膜缺血再灌注后MDA和NO水平的升高,同时能改善平均视网膜内层厚度的变化,但对SOD的作用不确定。结论牛磺酸可减轻大鼠视网膜缺血再灌注后的损伤。
Objective To observe the effect and mechanism of taurine on retinal ischemia-reperfusion (RIR) in rats. Methods 120 rats were randomly divided into three groups: control group, ischemia group and protection group. The intraocular perfusion liquid was used to form intraocular pressure (IOP) of 14.63 kPa for 1 h. The RIR model was established for 3 days and 2 times / d , Intraperitoneal injection of 10% taurine, a dose of 100 mg / kg, filling 30 min before surgery 1 time. The control group was given the same dose of saline. Two groups of ischemia 60 min, respectively, reperfusion 0,2,6,12,24,48,72 h colorimetric determination of retinal SOD, MDA, NO; measured with embedded microscope, the average intra-retinal section The mean thickness of inner retinal layer (MTIRL). Results Taurine could significantly antagonize the increase of MDA and NO levels after retinal ischemia-reperfusion in rats, meanwhile, it could ameliorate the change of mean retinal inner layer thickness, but its effect on SOD was uncertain. Conclusion Taurine attenuates retinal ischemia-reperfusion injury in rats.