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目的:探讨转化生长因子β1(TGF-β1)在原发性肝癌组织中的表达及其与肿瘤侵袭和转移的关系。方法:选择288例确诊为原发性肝癌患者作为研究组,另选取274例健康志愿者作为对照组。应用酶联免疫吸附法检测两组研究对象血清TGF-β1水平,免疫组织化学标记法检测肝癌患者肿瘤组织、癌旁组织及正常肝脏组织中TGF-β1的表达情况,并结合临床病理资料进行相关性分析。结果:原发性肝癌患者血清中TGF-β1水平明显高于对照组,差异具有统计学意义(P<0.01)。血清TGF-β1水平与肿瘤的组织学分级、浸润深度及转移呈正相关(P<0.01)。肝癌患者肿瘤组织中TGF-β1表达水平明显高于正常肝脏组织及癌旁组织,差异具有统计学意义(P<0.01)。结论:血清TGF-β1与原发性肝癌的侵袭和转移密切相关。TGF-β1可能会改变肿瘤微环境,增加侵袭和转移的潜力。
Objective: To investigate the expression of transforming growth factor β1 (TGF-β1) in primary hepatocellular carcinoma and its relationship with tumor invasion and metastasis. Methods: A total of 288 patients with primary liver cancer were selected as study group, and 274 healthy volunteers were selected as control group. The level of serum TGF-β1 was detected by enzyme-linked immunosorbent assay (ELISA). The expression of TGF-β1 in tumor tissues, adjacent non-cancerous tissues and normal liver tissues was detected by immunohistochemical method and correlated with clinicopathological data Sexual analysis. Results: The serum levels of TGF-β1 in patients with primary liver cancer were significantly higher than those in the control group (P <0.01). Serum levels of TGF-β1 were positively correlated with histological grade, depth of invasion and metastasis (P <0.01). The expression of TGF-β1 in hepatocellular carcinoma tissue was significantly higher than that in normal liver tissue and paracancerous tissue, the difference was statistically significant (P <0.01). Conclusion: Serum TGF-β1 is closely related to the invasion and metastasis of primary liver cancer. TGF-β1 may alter the tumor microenvironment and increase the potential for invasion and metastasis.