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探讨VEGF在膀胱肿瘤生长和转移中的作用,实验结果表明,反义VEGF121表达质粒转染能够明显降低肿瘤细胞产生VEGF.稳定转染的克隆其VEGF的表达量仅为未转染的亲代细胞的12％及19％.低水平表达VEGF的肿瘤细胞在体外的生长速度与未转染的亲代细胞间无明显差异,但在同系小鼠体内的致瘤能力、生长速度及肺、肝等实质器官的转移率等均明显低于亲代细胞.进一步比较高水平与低水平表达VEGF的肿瘤细胞对体外培养的小鼠血管内皮细胞生长的影响、体内肿瘤组织中微血管数目及通透性等,证实膀胱癌细胞产生VEGF的能力与其诱导肿瘤新生血管形成的能力一致,在肿瘤生长与转移过程中起重要作用.
To investigate the role of VEGF in the growth and metastasis of bladder tumor. The results showed that transfection of antisense VEGF121 expression plasmid could significantly reduce the production of VEGF in tumor cells. The stably transfected clones showed only VEGF expression in untransfected parental cells 12%, and 19% respectively.The tumor growth rate in vitro was not significantly different between low-level VEGF-expressing tumor cells and non-transfected parental cells, but tumorigenicity, growth rate, Were significantly lower than that of parental cells.Comparing the effect of high and low levels of VEGF expressing tumor cells on the growth of vascular endothelial cells cultured in vitro, the number of microvessels in tumor tissue and the permeability in vivo, it was confirmed that bladder The ability of cancer cells to produce VEGF consistent with their ability to induce neovascularization of tumor plays an important role in tumor growth and metastasis.