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目的探讨慢性间歇性缺氧条件下兔海马区脑组织磷酸化血管表皮生长因子(P-VEGFR)的蛋白质及mRNA表达及临床意义。方法通过建立不同组兔慢性间歇性缺氧模型,A组:空白对照组;B组:间歇性缺氧8周,撤销4周;C组:间歇性缺氧12周,后4周加上药物干预;D组:间歇性缺氧12周。应用Western Blot定量检测各组兔海马组织P-VEGFR蛋白质水平表达,并应用RT-PCR(reverse transcriptase polymerase chainreaction)方法半定量检测各组兔海马组织中的mRNA水平表达。结果 A组P-VEGFR蛋白质与mRNA的表达显著低于其他各组(P<0.05);B组、C组P-VEGFR的蛋白质与mRNA表达均显著低于D组(P<0.05);而B组与C组之间差异无统计学意义(P>0.05)。结论 (1)P-VEGFR的表达可能与氧化应激相关;(2)提示机体可能通过上调P-VEGFR的表达来抵抗慢性间歇性缺氧造成的脑组织损伤;(3)他汀类药物干预提示可能对兔海马缺氧性损害有一定的治疗作用。
Objective To investigate the expression and clinical significance of phosphorylation of vascular endothelial growth factor (P-VEGFR) in the hippocampus of chronic intermittent hypoxia. Methods: The chronic intermittent hypoxia model was established in different groups of rabbits. Group A: blank control group; group B: intermittent hypoxia for 8 weeks, withdrawal for 4 weeks; group C: intermittent hypoxia for 12 weeks, Intervention; Group D: intermittent hypoxia for 12 weeks. Western Blot was used to quantitatively detect the expression of P-VEGFR protein in hippocampus of each group. Semi-quantitative RT-PCR (reverse transcriptase polymerase chain reaction) method was used to detect the mRNA expression of hippocampus in each group. Results The protein and mRNA expression of P-VEGFR in group A were significantly lower than those in other groups (P <0.05). The protein and mRNA expressions of P-VEGFR in group B and group C were significantly lower than those in group D (P <0.05) There was no significant difference between group and group C (P> 0.05). Conclusions (1) The expression of P-VEGFR may be related to oxidative stress; (2) It suggests that the body may resist brain injury caused by chronic intermittent hypoxia by up-regulating the expression of P-VEGFR; (3) May have some therapeutic effect on hippocampal hypoxic damage in rabbits.