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目的探讨酶联免疫法检测小儿乙肝病毒前S1蛋白(PreS1)抗原及抗体在小儿乙肝诊断中的应用价值。方法在2014年至2016年在本院接受诊断和治疗的乙肝患儿中,选择200例资料完整且接受两对半检查和酶联免疫法检测血清PreS1抗原及抗体的患儿作为研究对象,计算不同两对半检测结果患儿PreS1抗原及抗体阳性率,分析PreS1抗原阳性与HBV-M的Spearman相关性。结果 200例乙肝患者入选。18例乙肝大三阳患儿的PreS1抗原阳性及抗体阳性的检出率较高,均为83.33%。25例PreS1抗原阳性组患儿与175例抗原阴性组患儿HBsAg、HBeAg和HBcAb数据对比差异具有统计学意义,说明PreS1抗原阳性与HBsAg、HBeAg和HBcAb具有正相关关系,相关数值分别为0.620、0.550、0.720,P值分别为0.021、0.009、0.020。结论乙肝PreS1抗原及抗体阳性检测准确率相对较高,可以作为乙肝诊断的补充检验,为患儿的临床诊断和治疗提供依据。
Objective To investigate the diagnostic value of PreS1 antigen and antibody in children with hepatitis B virus by enzyme-linked immunosorbent assay. Methods A total of 200 children with HBV diagnosed and treated in our hospital from 2014 to 2016 were enrolled in this study. Two hundred and seventy children with complete data and serum PreS1 antigen and antibody detected by two-and-one-half examination and enzyme-linked immunosorbent assay PreS1 antigen and antibody positive rate in children with different two pairs and a half test results, Spearman correlation between PreS1 antigen positive and HBV-M was analyzed. Results 200 cases of hepatitis B patients were enrolled. The positive rate of PreS1 antigen and antibody positive in 18 cases of HBeAg positive were higher, which were both 83.33%. There were significant differences in the data of HBsAg, HBeAg and HBcAb between 25 PreS1 positive patients and 175 negative patients, indicating a positive correlation between PreS1 positive HBsAg, HBeAg and HBcAb, the correlation values were 0.620, 0.550,0.720, P values were 0.021,0.009,0.020. Conclusion The positive detection rate of PreS1 antigen and antibody in hepatitis B is relatively high, which can be used as a supplementary test for the diagnosis of hepatitis B and provide the basis for the clinical diagnosis and treatment of children.