论文部分内容阅读
用毛细管区带电泳间接紫外检测法分离测定６种甲基膦酸单酯。以苯基膦酸为紫外添加剂，检测波长为 ２１０ ｎｍ。优化后缓冲溶液组成为：２００ ｍｍｏｌ／Ｌ硼酸－５ ｍｍｏｌ／Ｌ苯基膦酸－０．１０ ｍｍｏｌ／Ｌ ＤＤＡＢ－０．２０％Ｔｒｉｔｏｎ Ｘ－１００（ｐＨ ３．５５）。毛细管规格为７０ ｃｍ ｘ ７５ μｍ ｉ．ｄ。以ＫＨ２ｎＰＯ４为内标定量，在１～３０ｍｇ／Ｌ浓度范围内６种甲基膦酸单酯标准回归曲线线性关系良好（ｒ＞０．９９８９），检测限为０．２５～０．５ｍｇ／Ｌ。总分析时间在１４ ｍｉｎ以内。
Six kinds of methylphosphonic acid monoesters were separated and determined by capillary zone electrophoresis with indirect UV detection. Phenylphosphonic acid as UV additive, detection wavelength of 210 nm. The optimized buffer solution consisted of 200 mmol / L boric acid - 5 mmol / L phenylphosphonic acid - 0.10 mmol / L DDAB - 0.20% Triton X-100 (pH 3.55). Capillary specifications are 70 cm x 75 μm i. d. KH2nPO4 was used as internal standard to quantify the standard curve of 6 methylphosphonic acid monoesters with good linearity (r> 0.9989) within the range of 1 ~ 30mg / L with the detection limit of 0.25 ~ 0.5mg / L . The total analysis time is within 14 min.