垂体腺瘤p16纯和性缺失的研究

• 作 者: 魏新亭 李建章 罗世祺
• 机 构: 河南医科大学第一附属医院神经外科!郑州450052,河南医科大学第二附属医院神经内科!郑州450003,北京天坛医院神经外科!北京100050
• 出 处: 河南实用神经疾病杂志
• 发表日期: 2000年06期
• 关键词: p16 侵袭性垂体腺瘤 纯合性缺失 p16纯合性缺失 非侵袭性 分子标志 探针 核酸杂交技术 DNA 放射自显影

目的 :探讨p16的纯合性缺失与垂体腺瘤发生和侵袭的关系。对象与方法 :临床MRI和病理证实的垂体腺瘤标本 40例 ,DNA提取后酶切 ,采用核酸杂交技术与同位素标记的p16cDNA探针进行杂交 ,放射自显影显示结果。结果 :p16纯和性缺失的病例 ,其MRI表现为侵袭性垂体腺瘤 ,而非侵袭性垂体腺瘤则未发现有纯和性缺失。结论 :垂体腺瘤的发生与p16的表达缺失有关 ,而p16基因的纯和性缺失是侵袭性垂体腺瘤的分子标志之一。

Objective: To investigate the relationship between loss of homozygosity of p16 and the occurrence and invasion of pituitary adenoma. Subjects and Methods: Clinical MRI and pathological examination of 40 cases of pituitary adenoma specimens, DNA extraction after digestion, using nucleic acid hybridization and isotope-labeled p16 cDNA probe hybridization, autoradiography show the results. Results: The MRI findings of invasive pituitary adenomas were in the absence of p16 homozygosity and non-invasive pituitary adenomas. Conclusions: The occurrence of pituitary adenomas is related to the loss of p16 expression, and the lack of p16 gene homogeneity is one of the molecular markers of invasive pituitary adenomas.