【摘 要】
：
Propionate is a short-chain fatty acid(SCFA)mainly produced from carbohydrates by gut microbiota.Sodium propionate(SP)has shown to suppress the invasion in G protein-coupled receptor 41(GPR41)and GPR43-overexpressing breast cancer cells.In this study we i
【机 构】
：
Department of Pharmacology,Chungnam National University College of Pharmacy,Daejeon 34134,Republic o
论文部分内容阅读
Propionate is a short-chain fatty acid(SCFA)mainly produced from carbohydrates by gut microbiota.Sodium propionate(SP)has shown to suppress the invasion in G protein-coupled receptor 41(GPR41)and GPR43-overexpressing breast cancer cells.In this study we investigated the effects of SP on the proliferation,apoptosis,autophagy,and antioxidant production of breast cancer cells.We showed that SP(5-20 mM)dose-dependently inhibited proliferation and induced apoptosis in breast cancer cell lines JIMT-1(ER-negative and HER2-expressing)and MCF7(ER-positive type),and this effect was not affected by PTX,thus not mediated by the GPR41 or GPR43 SCFA receptors.Meanwhile,we demonstrated that SP treatment increased autophagic and antioxidant activity in JIMT-1 and MCF7 breast cancer cells,which might be a compensatory mechanism to overcome SP-induced apoptosis,but were not sufficient to overcome SP-mediated suppression of proliferation and induction of apoptosis.We revealed that the anticancer effect of SP was mediated by inhibiting JAK2/STAT3 signaling which led to cell-cycle arrest at G0/G1 phase,and increasing levels of ROS and phosphorylation of p38 MAPK which induced apoptosis.In nude mice bearing JIMT-1 and MCF7 cells xenograft,administration of SP(20 mg/mL in drinking water)significantly suppressed tumor growth by regulating STAT3 and p38 in tumor tissues.These results suggest that SP suppresses proliferation and induces apoptosis in breast cancer cells by inhibiting STAT3,increasing the ROS level and activating p38.Therefore,SP is a candidate therapeutic agent for breast cancer.
其他文献
Proteasome inhibitors,bortezomib(BTZ),and carfilzomib(CFZ)are approved drugs for hematological malignancies,but lack anticancer activities against most solid tumors.Small cell lung cancer(SCLC)is a very aggressive neuroendocrine carcinoma of the lungs dem
Aflibercept,as a soluble decoy vascular endothelial growth factor receptor,Which has been used as a first-line monotherapy for cancers.Aflibercept often causes cardiovascular toxicities including hypertension,but the mechanisms underlying aflibercept-indu
Hemorrhagic transformation(HT)is a common serious complication of stroke after thrombolysis treatment,which limits the clinical use of tissue plasminogen activator(t-PA).Since early diagnosis and treatment for HT is important to improve the prognosis of s
Oxidative stress is intimately tied to neurodegenerative diseases,including Parkinson\'s disease and amyotrophic lateral sclerosis,and acute injuries,such as ischemic stroke and traumatic brain injury.Acid sensing ion channel 1a(ASIC1a),a proton-gated i
目的 分析阜阳市首例人感染H5N6禽流感轻症患儿流行病学特征,为做好病例诊治及防控工作提供参考.方法 调查人感染H5N6禽流感患儿发病就诊情况、密切接触者、可疑禽类及活禽市场环境情况,采集患儿及其涉及的人群、活禽和环境标本并进行流感病毒检测,应用描述性流行病学方法进行分析.结果 阜阳市1例人感染H5N6禽流感患儿为流感常规监测发现,发病约10 h服用磷酸奥司他韦,约20 h症状消失,密切接触者无感染,可疑活禽摊位环境标本检测出现H5型禽流感病毒.结论 患儿有活禽市场环境暴露史,早发现早治疗,对减轻病情,提
Voltage-gated sodium channel Nav1.7 robustly expressed in peripheral nociceptive neurons has been considered as a therapeutic target for chronic pain,but there is no selective Nav1.7 inhibitor available for therapy of chronic pain.Ralfinamide has shown an
目的 采用指数平滑法预测艾滋病新报告病例的趋势,为泸州市制定预防和控制艾滋病策略提供依据.方法 根据2018-2019年泸州市每月艾滋病新报告病例资料,使用指数平滑法构建预测模型,预测2020年上半年每月新报告病例,并对预测结果进行评价.结果 综合比较指数平滑法四种模型的判断指标,确定Holt线性趋势模型为最优预测模型,模型的拟合度较高,实际值均在预测值的95％置信区间内.2020年1～6月艾滋病新报告病例数和模型预测值的平均相对误差33.19％.结论 Holt线性趋势模型可较好地拟合泸州市艾滋病新报告病
Acute liver failure(ALF)is a fatal clinical syndrome with no special drug.Recent evidence shows that modulation of macrophage to inhibit inflammation may be a promising strategy for ALF treatment.In this study we investigated the potential therapeutic eff
Brexpiprazole(Bre)is a new multi-target antipsychotic drug(APD)approved by the US FDA in 2015,and shows good therapeutic potential.But it lacks assessments on the metabolic side effects,which obstructs the treatment of schizophrenia.Glucagon-like peptide
Epithelial-mesenchymal transition(EMT)enables dissemination of neoplastic cells and onset of distal metastasis of primary tumors.However,the regulatory mechanisms of EMT by microenvironmental factors such as transforming growth factor-β(TGF-β)remain large