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2016年4月份,山东省某公司购买的拟做猪瘟脾淋苗试验的兔子出现以发病急、死亡快,口鼻出血,肺脏出血、淤血为特征的疾病,根据发病、死亡兔的临床表现、剖检病变,初步怀疑为兔巴氏杆菌或兔支气管败血波氏杆菌感染,取肺脏组织进行细菌的分离和培养、玻片凝集试验进行验证;对培养的菌落进行16S、巴氏杆菌(Pm)基因和波氏杆菌(Bb)基因的PCR扩增,并克隆、测序。确诊该批兔是感染了兔支气管败血波氏杆菌。
In April 2016, a rabbit purchased from a company in Shandong Province to be used to make swine fever and spleen-spleen leaching test showed the disease characterized by rapid onset, rapid death, nose and throat bleeding, pulmonary hemorrhage and congestion. According to the clinical manifestations of the deceased rabbits , Pre-test lesions, initially suspected of Pasteurella or rabbit bronchiseptica infection, take the lung tissue isolation and culture of bacteria, slide agglutination test to verify; cultured colonies 16S, Pasteurella ( Pm) gene and Bordetella (Bb) gene PCR amplification, and cloned and sequenced. Rabbits diagnosed with this group of rabbits are infected with Bordetella bronchiseptica.