论文部分内容阅读
目的:研究复方当归汤中阿魏酸在兔体内的药物动力学规律。方法:采用兔按4ml·kg~(-1)单次灌胃给予复方当归汤,于不同时间点取样,不测定血浆中阿魏酸浓度,并用3P87软件处理药物动力学参数。采用RP-HPLC测定血药浓度,以甲醇-水(0.3%冰醋酸)(40:60)为流动相,Zorbax SBC_(18)(150 mm×4.6 mm,5μm),紫外检测波长323 nm。结果:兔口服复方当归汤的血浆药-时曲线表现为单峰二室模型,主要药物动力学参数如下:Ka(min~(-1))=0.1400,K_(21)=1.5×10~(-2),K_(10)=1.1×10~(-2),K_(12)=3.4×10~(-2),t_(1/2α)(min)=12.05,t_(1/2β)(min)=224.45,t_(1/2γ)(min)=5.11,C_(max)(ng·ml~(-1))=73.20,AUC(ng·min~(-1)·ml)= 1.082×10~4,CL/F(s)(L·min~(-1))=3.700×10~(-4),V/F(c)(ng·ml~(-1))=3.2×10~(-2)。结论:复方当归汤的体内有效成分阿魏酸吸收分布呈二室模型。
Objective: To study the pharmacokinetics of compound ferulic acid in rabbits. Methods: The rabbits were fed with Danggui Decoction by single-dose 4ml·kg -1 . The samples were taken at different time points. The concentration of ferulic acid in the plasma was not determined. The pharmacokinetic parameters were treated with 3P87 software. The plasma concentration was determined by RP-HPLC. The mobile phase was methanol-water (0.3% glacial acetic acid) (40:60), Zorbax SBC_(18) (150 mm×4.6 mm, 5 μm), and UV detection wavelength was 323 nm. RESULTS: The plasma drug-time curve of compound Angelica Decoction in rabbits was shown as a unimodal two-compartment model. The main pharmacokinetic parameters were as follows: Ka(min-1)=0.1400, K21=1.5×10- -2), K_(10)=1.1×10~(-2), K_(12)=3.4×10~(-2), t_(1/2α)(min)=12.05, t_(1/2β) (min)=224.45, t_(1/2γ)(min)=5.11, C_(max)(ng·ml~(-1))=73.20, AUC(ng·min~(-1)·ml)=1.082 ×10~4, CL/F(s)(L·min~(-1))=3.700×10~(-4), V/F(c)(ng·ml~(-1))=3.2× 10~(-2). Conclusion: The composition of ferulic acid absorbed in the body of compound Angelica Decoction is a two-compartment model.