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采用进口美麦中截获的单个、经萌发的TilletiacontroversaK櫣ｈｎ(TCK)冬孢子人工接种创伤和未创伤的美国硬红冬小麦的胚芽鞘 ,每个胚芽鞘接种一个萌发TCK冬孢子 ,接种后麦苗在 5℃、1 2h光照和黑暗交替条件下培养 2 1或 2 9天 ,然后移植到隔离网室中的塑料周转箱中作常规栽培管理 ,结果均得到了TCK病穗。这是单个萌发TCK冬孢子引起小麦发病的首次报道。本试验结果表明 ,在自然条件下 ,当寄主和环境条件适宜时 ,微量的甚至单个TCK冬孢子就能引起小麦发病。这为开展TCK在我国的定殖可能性研究提供了一定的实验依据。
A single, germinated Tilletiacontroversa K hn (TCK) teliospores intercepted from imported M. malt were artificially inoculated with a coleoptile of both traumatized and non-traumatic United States hard red winter wheat. One germ sheath was inoculated with one germinating TCK teliospore, 5 ℃, 1 2h light and dark conditions for 21 days or 29 days, and then transplanted into the plastic mesh cage isolation room for routine cultivation and management, the results have been TCK panicle disease. This is the first report of a single germination of wheat germ caused by TCK teliospores. The results of this experiment show that in natural conditions, trace amounts of even a single TCK teliospore can cause wheat disease when host and environmental conditions are appropriate. This provides a certain experimental basis for carrying out TCK research on the possibility of colonization in our country.