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发展能实时检测完整细胞内多个生物分子随时空变化的单分子探测和追踪技术,对于研究生命过程的分子机理具有重要意义.在变形光栅多阶成像和双螺旋点扩散函数成像方法的基础上,基于波前编码的原理,提出将二者优化结合,获得全新的衍射光学元件,该器件同时具有多重平面成像和双螺旋点扩散函数成像的功能,旨在解决活细胞内单分子探测和追踪技术中的大景深探测难题.设计和制备了该器件,并基于该器件搭建了显微成像系统,实验模拟证明该衍射光学元件同时可实现轴向12μm的探测范围,与理论设计结果相符合,从而有效扩大了显微镜系统的景深,证明了设计的可行性.
The development of single-molecule detection and tracing technology that can detect the temporal and spatial changes of multiple biomolecules in intact cells in real time is of great significance for studying the molecular mechanism of life processes.Based on the multi-stage imaging of deformed gratings and the imaging method of double helix point spread function , Based on the principle of wavefront coding, a new diffractive optical element based on the principle of wavefront coding is proposed. The new diffractive optical element is proposed. It has the functions of multiple planar imaging and double helix point spread function imaging, and aims to solve single molecule detection and tracking in living cells Technology, the device has been designed and fabricated, and a microscopic imaging system has been built based on the device. The experimental simulation proves that the diffractive optical element can realize the detection range of 12 μm in the axial direction at the same time, which is consistent with the theoretical design results. Thus effectively expanding the depth of field of the microscope system, proving the feasibility of the design.