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春小麦是青海省的主要粮食作物,青海高原干旱频繁且严重,尤以春旱为首,对小麦生长发育造成严重影响.为了从蛋白质组学水平分析小麦对干旱胁迫的应答特征,探讨小麦可能的抗旱机制,本研究对青海主栽春小麦品种青春38幼苗进行聚乙二醇(polyethylene glycol,PEG)6000胁迫和复水处理,采用IEF/SDS-PAGE双向凝胶电泳技术,对PEG胁迫和复水处理的小麦叶片总蛋白质分别与正常浇水的对照进行差异蛋白质组学研究,经考马斯亮蓝G-250染色获得清晰度和重复性较好的双向电泳图谱.PD Quest软件处理分析各对照和处理图谱,在等电点4.0～7.0线性范围内,均可识别650个以上清晰蛋白质点,获得比较明显的差异表达蛋白点43个,其中8个蛋白点重复.从35个差异蛋白点中选取24个差异点进行MALDI-TOF-TOF-MS肽质量指纹图谱分析,应用Mascot软件在NCBInr数据库中搜索鉴定蛋白质,得到22个阳性结果.对鉴定得到的差异表达蛋白进行功能分析,它们分别参与了光合作用、蛋白质合成、能量代谢途径、细胞防御、氧化还原、运输、信号转导等过程,而且根据差异表达蛋白功能分类所占比例,发现干旱胁迫与光合作用关系最为紧密.
Spring wheat is the main food crop in Qinghai Province, and the drought in the Qinghai Plateau is frequent and serious, especially in the spring drought, which has a serious impact on the growth and development of wheat.In order to analyze the response characteristics of wheat to drought stress at proteomics level, Mechanism, we studied the effects of polyethylene glycol (PEG) 6000 stress and rewatering treatment on spring wheat seedlings of main cultivated Qinghai 38 in Qinghai. By means of IEF / SDS-PAGE two-dimensional gel electrophoresis, the effects of PEG stress and rehydration Of the total protein of wheat leaves were compared with the normal watering control differential proteomics study, by Coomassie Brilliant Blue G-250 staining obtained better resolution and repeatability of the two-dimensional electrophoresis .PD Quest software processing and analysis of each control and treatment map , More than 650 clear protein spots were identified in the linear range of isoelectric points from 4.0 to 7.0, and 43 differentially expressed protein spots were obtained, in which 8 spots were duplicated.From 24 spots of 35 spots Differences were analyzed by MALDI-TOF-TOF-MS peptide mass fingerprinting, and Mascot software was used to search the NCBInr database to identify the proteins and obtain 22 positive results. They were involved in the processes of photosynthesis, protein synthesis, energy metabolism pathways, cell defense, redox, transport, signal transduction and so on, and according to the proportion of differentially expressed protein functional classification, found that Drought stress is most closely related to photosynthesis.