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七宝美髯丹为我国侍统养生名方。现将实验研究报道如下。材料与方法一、药物:制首乌1000g、牛膝、菟丝子、当归、补骨脂、枸杞子、茯苓各250g,经严格的传统炮制方法研末备用。二、动物:200±20g雌大鼠。22±4g雌小鼠。两种动物均随机分为给药组(基础饲料中掺入等比例药末)与对照组(只给足量基础饲料)。三、方法:分组饲养15日后,分别进行应激试验,血红蛋白(Hb)、血清铁与过氧化氢酶(CAT)测定。应激试验选取耐缺氧为代表,在等负压抽滤瓶中,观察小鼠在缺氧状况下的应激生存能力,直至窒息死亡并记录死亡时间。大鼠Hb与血清铁按临床常规方法于剪尾取血后检测,小鼠血浆中CAT按碘量法测定其活力单位。
Qibao Meilan Dan is the Chinese name for his regimen. Experimental studies are now reported as follows. Materials and methods First, drugs: Shouwu 1000g, Achyranthes, dodder, angelica, psoralen, wolfberry fruit, wolfberry each 250g, after the strict traditional methods of processing into spare. Second, animals: 200 ± 20g female rats. 22±4g female mice. Both animals were randomly divided into the administration group (mixed with the proportion of the drug in the basal diet) and the control group (only sufficient basal diet). 3. Methods: After 15 days of group feeding, stress tests were performed, and hemoglobin (Hb), serum iron, and catalase (CAT) were measured. In the stress test, hypoxia tolerance was selected as the representative. In hypotonic suction flasks, the mice were observed for stress survival under hypoxic conditions until suffocation died and the time of death was recorded. Rats’ Hb and serum iron were detected by conventional routine methods after blood collection by tail-clamping, and CAT in mouse plasma was measured by iodometric method.