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多种致病因子长期作用均可致肝纤维化,其形成是细胞外基质(extracellular matrix,ECM)产生和降解不平衡的结果.星状细胞(hepatic stellate cell,HSC)活化是肝纤维化发生的中心环节.缺氧是最常见的致病或伴随因素之一,其对 HSC 产生胶原及胶原降解酶有何影响尚不清楚.作者对原代 HSC 行缺氧培养,用 ELISA 方法测培养上清中基质金属蛋白酶1,2(MMP-1,MMP-2)、Ⅳ型胶原(collagenase Ⅳ,Col Ⅳ)及Ⅲ型前胶原氨基端肽(PⅢP)相对含量.结果报道如下,1 材料和方法1.1 材料Ⅳ型胶原酶、Pronase E,Nycodenz,DMEM 培养基、DMEM/F12(v/v=1/1)无血清培养基为 Sigma 产品;MMP-1
Long-term effects of a variety of causative agents can cause hepatic fibrosis, which is the result of an imbalance in the production and degradation of the extracellular matrix (ECM), and activation of hepatic stellate cells (HSC) Of hypoxia is one of the most common pathogenicity or accompanying factors.It is not clear whether HSC can produce collagen and collagen degrading enzymes.The hypoxic culture of primary HSC was measured by ELISA The relative contents of MMP-1, MMP-2, Col Ⅳ and PⅢP in serum were reported in this paper.1 Materials and Methods 1.1 Materials Type IV collagenase, Pronase E, Nycodenz, DMEM medium, DMEM / F12 (v / v = 1/1) serum-