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以牡丹‘藏枝红’为试材,研究了柱枝孢叶斑病(Cylindrocladium canadense)侵染对牡丹生理特性的影响。结果表明:病原菌侵染后牡丹叶片净光合速率(Pn)和光合色素含量显著降低,同时表观量子效率和羧化效率均显著下降,表明侵染增强了牡丹叶片的光抑制;C.canadense侵染后,抗氧化酶活性发生显著变化,超氧化物歧化酶(SOD)和过氧化氢酶(CAT)活性在侵染5d先略有升高,然后呈显著下降趋势;过氧化物酶(POD)和抗坏血酸过氧化物酶(APX)活性一直呈下降趋势;O·2产生速率和H2O2积累量明显增加,丙二醛(MDA)含量显著升高。C.canadense侵染使抗氧化酶活性有不同程度的下降,导致活性氧(ROS)含量增加,对生物膜系统造成伤害,从而降低了牡丹叶片的光合能力。
The effect of Cylindrocladium canadense infection on the physiological characteristics of peony was studied using ’Tibetan red branch’ of peony. The results showed that the net photosynthetic rate (Pn) and photosynthetic pigment content of leaves of Peony were significantly decreased after infected by pathogen, while the apparent quantum yield and carboxylation efficiency decreased significantly, indicating that the infection enhanced the photoperiod of peony leaves; C. canadense invasion After dyeing, the activities of antioxidant enzymes changed significantly. The activity of superoxide dismutase (SOD) and catalase (CAT) increased slightly at the 5th day after infection, then decreased significantly. The activity of peroxidase ) And ascorbate peroxidase (APX) activity all showed a decreasing trend. The generation rate of O · 2 and the accumulation of H2O2 increased significantly, and the content of malondialdehyde (MDA) increased significantly. C. canadense infection reduced the activities of antioxidant enzymes to different extents, resulting in the increase of reactive oxygen species (ROS) and the damage to the biofilm system, thus reducing the photosynthetic capacity of tree peonies.