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常规的马铃薯综合培养基,用来转接猴头、黑木耳及某些平菇菌种,菌丝生长往往不够理想。为此,我们通过大量的实践,获得了棉籽皮、麸皮水煮液母种培养基配方,现将其制备方法介绍如下。配方:棉籽皮100克,麸皮50克,磷酸二氢钾3克,蛋白胨2克,硫酸镁1.5克,V_(B1) 10毫克,葡萄糖20克,琼脂20克,水1000毫升。制作方法:先将称好的棉籽皮用水洗干净,挤干水,放入麸皮,加适量水,放在火上加热。开锅后小火煮10~15分钟,过滤。滤液中加入琼脂,边加热边搅拌,待琼脂溶化后,再加入其他成
Conventional potato integrated medium, used to transfer hericium, black fungus and some mushroom strains, mycelial growth is often not ideal. To this end, we through a large number of practices, access to cottonseed husk, bran boiled liquid parent culture medium formula, now its preparation method is described below. Recipe: Cottonseed husk 100g, bran 50g, potassium dihydrogen phosphate 3g, peptone 2g, magnesium sulphate 1.5g, V_ (B1) 10mg, glucose 20g, agar 20g, water 1000ml. Production methods: First weighed cottonseed skin washed with water, squeezed dry water, add bran, add appropriate amount of water, on the heat on the fire. Boil small simmer 10 to 15 minutes, filtered. Agar added to the filtrate, while heating while stirring until the agar melted, then add other into