Effect of Temperature on Rice Starch Biosynthesis Metabolism at Grain-Filling Stage of Early Indica

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The sucrose content, starch content and the ratio of amylose to total starch and the activities of six key enzymes, sucrose synthase(SS) , soluble starch synthesis enzyme(SSS) , ADPG pyrophosphorylase (ADPG-Ppase) , granule-boundstarch synthase (GBSS), starch branching enzyme (SBE) and debranching enzyme (DBE) , which involved in starch synthesis metabolism of developing rice grains and effect of temperature on their activities were analyzed by using two early indica varieties Zhefu49 and Jiazao935 in phyto-trons, the daily average temperature was 22 and 32℃, respectively. Results showed that the sucrose content, the starch content, and the activities of enzymes such as SS, ADPG-Ppase, SSS, SBE, and DBE were generally higher under the high temperature treatment (32℃)at the beginning stage as compared with that under low temperature treatment (22℃). However, at the middle and late stages, there were great differences in the change trend of some enzyme activities, and the association with the sucrose c The sucrose content, starch content and the ratio of amylose to total starch and the activities of six key enzymes, sucrose synthase (SS), soluble starch synthesis enzyme (SSS), ADPG pyrophosphorylase (ADPG- Ppase), granule- boundstarch synthase ), starch branching enzyme (SBE) and debranching enzyme (DBE), which involved in starch synthesis metabolism of developing rice grains and effect of temperature on their activities were analyzed by using two early indica varieties Zhefu 49 and Jiazao 935 in phyto-trons, the daily Results showed that the sucrose content, the starch content, and the activities of enzymes such as SS, ADPG-Ppase, SSS, SBE, and DBE were generally higher under the high temperature treatment (32 ℃) at the beginning stage as compared with that under low temperature treatment (22 ° C). However, at the middle and late stages, there were great differences in the change trend of some enzyme activities, and the associati on with the sucrose c
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