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有些导电高聚物如聚吡咯(PPy),聚苯胺等的电导率在一定范围内随着外部施加电压改变而变化,利用这一性质可以制作各种聚合物分子器件。我们曾用阵列微电极在水溶液中研究了PPy的电导率与外压的关系,发现这种PPy修饰的阵列微电极具有类似二极管的特性,并测出了其响应的I-V曲线。但将生物酶固定在阵列微电极上制成特定的生物电化学分子器件还未见文献报道。由于这种分子器件能制成传感器,用于临床化学、环境监测、过程控制及生命体系分析中,因此有必要开展这方面的研究。本文用PPy将硫辛酰胺脱氢酶(DP)固定在对插型阵列微电极(IDA)上,研究了PPy/DP修饰IDA电极(PPy/DP/IDA)的二极管特性,测出了其I_D-V_G曲线,发现烟酰胺腺嘌呤二核苷酸(NADH)能使PPy/DP/IDA分子器件从“开”状态变到“关”状态,并且其“开～关”时间与NADH的浓度有关并可用于对NADH的定量测定。本文的这一发现对进一步研究和制作新型的NADH生物电化学传感器具有指导意义。
Some conductive polymers such as polypyrrole (PPy), polyaniline, etc. conductivity within a certain range with the external applied voltage changes, the use of this property can produce a variety of polymer molecular devices. We have studied the relationship between PPy conductivity and external pressure using an array microelectrode in aqueous solution. We found that PPy-modified array microelectrode has diode-like behavior and measured the I-V curve of its response. However, it has not been reported in the literature that the biological enzyme is immobilized on an array microelectrode to make a specific bioelectrochemical molecular device. Since this molecular device can be used as a sensor in clinical chemistry, environmental monitoring, process control and life system analysis, it is necessary to carry out this research. In this paper, PPy was used to immobilize lipoamide dehydrogenase (DP) on the arrayed array microelectrode (IDA). The diode characteristics of PPy / DP modified IDA electrode (PPy / DP / IDA) -V_G curve. It was found that nicotinamide adenine dinucleotide (NADH) can change PPy / DP / IDA molecular device from “on” state to “off” state and its “on-off” time is related to the concentration of NADH And can be used for quantitative determination of NADH. This discovery of this article is of guiding significance for further research and production of new NADH bioelectrochemical sensors.