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研究了细胞色素Ｃ（Ｃｙｔ．Ｃ）在一种新的电子转移促进剂４，６－二甲基－２－巯基嘧啶修饰微带金电极（ＤＭＭＰ／Ａｕ）上的氧化还原热力学；求得Ｃｙｔ．Ｃ在ＤＭＭＰ／Ａｕ电极上电化学反应的标准电极电位Ｅ￣０＇、熵变△Ｓ￣０、焓变△ＩＩ０及Ｇｉｂｂｓ自由能的改变△Ｇ￣０值分别为：０．２７２Ｖ（ｖｓ．ＮＨＥ）、－１２３．７Ｊ·ｍｏｌ￣（－１）·Ｋ￣（－１）、－６３．１ｋＪ·ｍｏｌ￣（－１）和－２６．２ｋＪ·ｍｏｌ￣（－１）（２５℃，ｐＨ＝７．０）；同时，探讨了温度及ｐＨ值对Ｃｙｔ．Ｃ电极反应热力学参数的影响．实验表明，碱性条件下Ｅ￣０＇随温度的变化表现出与中性及弱酸性条件下不同的规律．
The redox thermodynamics of cytochrome C (Cyt.C) on a novel electron transfer accelerator 4,6-dimethyl-2-mercaptopyrimidine modified microstrip gold electrode (DMMP / Au) was investigated. Cyt . The standard electrode potentials E ~ 0 ’, entropy change △ S ~ 0, enthalpy change △ II0 and Gibbs free energy △ C ~ 0 values of electrochemical reaction on DMMP / Au electrode were 0.272V (vs. NHE), -123.7 J · mol -1 (-1), -63.1 kJ · mol -1 and -26.2 kJ · mol -1 (25 ° C, pH = 7.0); At the same time, the temperature and pH on the Cyt. C electrode reaction thermodynamic parameters. Experiments show that under alkaline conditions E ~ 0 ’with temperature changes showed neutral and weak acid conditions under different laws.