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利用昌7-2与热带自交系CML451构建的BC1群体开花期、株高以及产量相关性状进行QTL定位分析。通过复合区间作图方法共检测到与散粉期、抽丝期、株高、穗位高、穗长、穗粗、穗轴粗、穗行数8个性状相关联的QTL 26个。控制散粉期的qDTP5被定位于第5染色体上的umc1523-bnlg1660标记区间,其解释了16%表型变异;控制株高的qPH3被定位于第3染色体上的bnlg1798-bnlg1852标记区间,解释了19.9%表型变异;控制穗位高的qEH10-2被定位于第10染色体上的umc1911-bnlg594标记区间,解释了13.4%表型变异;控制穗长的qEL10-2被定位于第10染色体的bnlg1250-bnlg594区间,解释了13%表型变异;控制穗粗的qED5、控制穗轴粗的qCD5、控制穗行数的qER5均被定位于第5染色体上的bnlg1660-bnlg1208区间,分别解释了18.1%、11.3%与21.8%表型变异;qED7定位于第7染色体上的umc1154-umc1799区间,与穗粗相关,解释了21.9%表型变异。
The QTL mapping of flowering, plant height and yield-related traits of BC1 population constructed by Chang 7-2 and CML451 inbred lines was conducted. A total of 26 QTLs associated with eight traits, including bolting stage, spinning stage, plant height, ear height, ear length, ear diameter, coarse cob and ear number, were detected by composite interval mapping method. The qDTP5 controlling the powdery phase was mapped on the umc1523-bnlg1660 marker interval on chromosome 5, which explained 16% of the phenotypic variation; the qPH3 controlling plant height was mapped on the bnlg1798-bnlg1852 marker interval on chromosome 3, accounting for 19.9 % Of phenotypic variation; qEH10-2 with high ear position was mapped on the umc1911-bnlg594 marker interval on chromosome 10, accounting for 13.4% of phenotypic variation; qEL10-2 controlling panicle length was mapped to bnlg1250 on chromosome 10 -bnlg594 interval, explaining 13% of phenotypic variation; qED5 controlling panicle coarse, qCD5 controlling panicle coarse, qER5 controlling panicle number were all mapped to bnlg1660-bnlg1208 interval on chromosome 5, respectively, explaining 18.1% , 11.3% and 21.8% respectively. QED7 was located on the umc1154-umc1799 interval on chromosome 7, which was related to the ear panicle, which explained 21.9% of the phenotypic variation.