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目的预测RhD抗体核酸适配体序列库中优势适配体,为实验室验证做准备。方法对以RhD抗体为目标靶分子而筛选出的适配体序列库做克隆测序;使用ClustalⅩ软件对阳性克隆子序列做同源性分析;使用DNA folding form在线分析工具对阳性克隆子碱基序列做最小自由能及二级结构分析。结果 RhD抗体28个阳性克隆子碱基序列按照基序的不同可分为3个家族,家族1和2中的适配体又可分为不同的亚家族;可形成由2-4个多分支环组成的二级结构,最小自由能在(-7.47--5.09)kcal/mol。结论 RhD家族1中的亚家族3和4适配体二级结构较为稳定,最小自由能较低,是RhD抗体适配体库中的优势适配体。
Objective To predict the dominant aptamers of RhD antibody aptamer library and prepare for laboratory validation. Methods The sequence of the aptamer library screened by RhD antibody was cloned and sequenced. The homology of the positive clones was analyzed by Clustal X software. The positive clonucleotide sequences were analyzed by the DNA folding form online analysis tool Do the minimum free energy and secondary structure analysis. Results The 28 positive cloned base sequences of RhD antibodies were divided into three families according to their motifs. The aptamers in families 1 and 2 were divided into different subfamilies. Secondary structure composed of rings, the minimum free energy in (-7.47 - 5.09) kcal / mol. Conclusion The secondary structure of subfamily 3 and 4 aptamers in RhD family 1 is more stable and has lower minimum free energy. It is the dominant aptamer in RhD antibody aptamer library.