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植物质膜水通道蛋白(plasma membrane intrinsic proteins,PIPs)是位于细胞质膜上具有选择性、高效转运水分的一类膜内在蛋白,参与植物生长发育的多个生理活动。本研究以大麦‘Haruna-nijo’为材料,对水培幼苗进行4℃冷胁迫,采用实时荧光定量PCR技术对胁迫期(4℃,48 h)和温度恢复期(16℃,48 h)两个过程的水通道蛋白PIPs基因表达进行了分析;同期测定了根水导度(Lpr)、根长和苗高,分析冷胁迫下大麦根PIPs基因的表达与水分生理的关系。结果表明:大麦幼苗经4℃低温胁迫48 h后,苗的生长明显受抑,根的生长无显著变化;温度恢复48 h后,苗恢复生长,根的生长无显著变化;根水导度在胁迫期下降,恢复期急剧升高,均无显著差异。实时荧光定量PCR结果显示,根中表达量最高的是HvPIP1;2和HvPIP1;3,最低的是HvPIP1;1和HvPIP2;3;冷处理后HvPIPs表达量与对照比较总体下降,其中HvPIP1;2、HvPIP1;3、HvPIP1;4、HvPIP1;5、HvPIP2;1、HvPIP2;2明显下调。恢复后大多数HvPIPs表达量增加,HvPIP1;1、HvPIP1;2、HvPIP1;5、HvPIP2;3显著增加,HvPIP1;4、HvPIP2;5表达量降低,但无显著差异。研究发现,冷胁迫后大麦根HvPIPs的表达情况总体下调,恢复生长大部分HvPIPs上调,结合根水导度的变化,推测大麦HvPIPs在抗冷反应中的作用复杂,冷害的不同阶段HvPIPs对水分吸收所起的作用不同。
Plant plasma membrane intrinsic proteins (PIPs) are a series of membrane intrinsic proteins that are located on the plasma membrane of the plasma membrane and efficiently transport water. They are involved in many physiological activities of plant growth and development. In this study, barley ’Haruna-nijo’ was used as the material to cold-stress the hydroponic seedlings at 4 ℃. The real-time PCR method was used to study the effects of stress (4 ℃, 48 h) Ppr and PIPs in barley roots under cold stress were analyzed. The relationship between PIPs gene expression and water physiology was analyzed. The results showed that the growth of seedlings was significantly inhibited and the growth of roots did not change significantly after barley seedlings were treated with low temperature stress of 4 ℃ for 48 h. After 48 h of temperature recovery, the seedlings grew and recovered without any significant changes in root growth. There was no significant difference in stress period and rapid recovery period. HvPIP1; 2 and HvPIP1; 3, the lowest HvPIP1; 1 and HvPIP2; 3; HvPIPs expression decreased compared with the control after cold treatment, of which HvPIP1; 2, HvPIP1 ; 3, HvPIP1; 4, HvPIP1; 5, HvPIP2; 1, HvPIP2; The expression of HvPIP1, HvPIP1; 2, HvPIP1; 5, HvPIP2; 3 increased significantly after the recovery, while the expression of HvPIP1; 4, HvPIP2; 5 decreased but there was no significant difference. The results showed that the expression of HvPIPs in barley roots was down-regulated after cold stress, and most of the HvPIPs recovered and up-regulated. Combined with the changes of root water conductivity, the effect of HvPIPs in barley cold resistance was complex. The role played by different.