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本文对用于生产Ｌ－苯丙氨酸的基因工程菌ＣＴＢ２的培养条件、催化苯丙酮酸生成Ｌ－苯丙氨酸的反应条件及其稳定性进行了研究。当苯丙酮酸钠盐浓度为２％，Ｌ－天冬氨酸浓度为５％，５－磷酸吡哆醛浓度为１００ｕｍｏｌ／Ｌ，溴化十六烷基三甲铵（ＣＴＡＢ）浓度为０．０３％，菌浓度为０．０２ｇ／ｍｌ，ｐＨ７．５，３０℃时，反应１小时，ＣＴＢ２菌催化苯丙酮酸转化为Ｌ－苯丙氨酸的转化率为８１％，生成Ｌ－苯丙氨酸的速度为１４．４ｇ／Ｌ．ｈ。
In this paper, the conditions for the cultivation of genetically engineered bacteria CTB2 for L-phenylalanine and the reaction conditions for the formation of L-phenylalanine from phenylpyruvate and their stability were studied. When the concentration of phenylpyruvate sodium was 2%, the concentration of L-aspartic acid was 5%, the concentration of pyridoxal 5-phosphate was 100 μmol / L, the concentration of cetyltrimethylammonium bromide (CTAB) was 0.03 %, Bacteria concentration of 0.02g / ml, pH7.5, at 30 ℃, the reaction for 1 hour, CTB2 bacteria catalyzed conversion of phenylpyruvate to L-phenylalanine was 81%, generating L-phenylalanine The speed of the acid was 14.4 g / L. h