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本工作采用原子吸收分光光度法测定侧脑室微量注射β－内啡肽（β－ＥＰ）、强啡肽Ａ（ＤｙｎＡ）和纳洛酮（Ｎｘ）后，对于沙土鼠急性缺血大脑皮层Ｃａ２＋、Ｍｇ２＋和Ｈ２Ｏ含量的影响；利用氢清除法观察了动物大脑皮层局部血流量以及静脉注射β－ＥＰ和Ｎｘ后，对于皮层局部血流量的影响，探讨β－ＥＰ和ＤｙｎＡ在沙土鼠急性缺血性大脑皮层水肿中的作用及作用机制。结果表明：侧脑室注射β－ＥＰ后，在缺血大脑皮层水肿加重的同时，大脑皮层Ｃａ２＋含量升高非常显著（Ｐ＜０．０１），而注射ＤｙｎＡ后，使皮层Ｃａ２＋含量明显降低（Ｐ＜０．０５）；侧脑室注射Ｎｘ后，沙土鼠缺血大脑皮层ｃａ２＋含量明显降低（Ｐ＜０．０１）的同时，皮层组织Ｍｇ２＋含量显著增加（Ｐ＜０．０５），缺血性大陆皮层水肿明显减轻（Ｐ＜０．０１）；尾静脉注射β－ＥＰ和Ｎｘ后，动物大脑皮层局部血流量和相应对照组相比没有显著改变。提示，β－ＥＰ加重或促进缺血性脑水肿的作用与大脑皮层局部血流量的改变无明显相关，内源性阿片肽在缺血性脑水肿中的作用及其差异可能和它们调节组织内Ｃａ２＋、Ｍｇ２＋代谢及Ｃａ２＋、Ｍｇ２＋含量的变化有关。
In this study, microinjection of β-EP, DynA and Nx into the lateral ventricle was performed by atomic absorption spectrophotometry for the determination of Ca2 +, Mg2 + and H 2 O. The effects of β-EP and DynA on the cerebral ischemia-reperfusion injury in gerbils were observed by using hydrogen scavenging method. The role and mechanism of cerebral cortex edema. The results showed that the intracerebroventricular (icv) injection of β-EP significantly increased the content of Ca2 + in the cerebral cortex (P <0.01), and decreased the content of Ca2 + in the cortex (P <0.05). After intracerebroventricular injection of Nx, the content of ca2 + in cerebral cortex of gerbils decreased significantly (P <0.01), while the content of Mg2 + in cortex increased significantly (P <0.05) Cortical edema was significantly reduced (P <0.01). After tail vein injection of β-EP and Nx, the cerebral blood flow in cerebral cortex did not change significantly compared with the corresponding control group. These results suggest that the role of β-EP in aggravating or promoting ischemic cerebral edema is not related to the change of local cerebral blood flow. The role and difference of endogenous opioid peptides in ischemic brain edema may be related to their role in the regulation of tissue Ca2 +, Mg2 + metabolism and Ca2 +, Mg2 + content changes.