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目的观察重伤后肺脏Zn含量及Cu/Zn-SOD活性与MDA含量的变化,探讨其与继发急性肺损伤的关系。方法参照ISS建立家兔重伤模型,检测伤后各组肺组织Zn水平、Cu/Zn-SOD活性、MDA含量变化,分析其与继发急性肺损伤的关系。结果重伤后,家兔出现急性肺损伤改变,以死亡组最为严重,肺Zn含量总体呈逐渐下降趋势,以伤后3、6d最低,死亡组显著降低(P<0.05);肺Cu/Zn-SOD活性在伤后1d降低,6d致低谷,并持续到第9d,死亡组显著降低(P<0.05);肺MDA在伤后3d升高,6d达高峰。死亡组MDA升高显著(P<0.05)。结论重伤可引发肺组织Zn水平及其Cu/Zn-SOD活性下降、MDA含量升高,该变化与继发急性肺损伤及死亡相关。
Objective To observe the changes of Zn content, Cu / Zn-SOD activity and MDA content in lungs of severely injured patients, and to explore its relationship with secondary acute lung injury. Methods Rabbit model of severe injury was established with reference to ISS. The level of Zn, the activity of Cu / Zn-SOD and the content of MDA in lung tissue of each group were detected after injury, and their relationship with secondary acute lung injury was analyzed. Results After acute severe injury, the acute lung injury in rabbits was the most serious in the death group. The content of Zn in the lung decreased gradually, and the lowest in the 3rd and 6th day (P <0.05) The activity of SOD decreased at 1 day after injury and reached the bottom at 6 days, and continued to the 9th day. The MDA level in the death group was significantly decreased (P <0.05). The MDA level in the lung increased 3 days after the injury and peaked on the 6th day. MDA in the death group increased significantly (P <0.05). Conclusions Severe injuries can lead to the decrease of Zn level and the decrease of Cu / Zn-SOD activity and the increase of MDA content in lung tissue, which is associated with secondary acute lung injury and death.