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目的探讨缺氧缺血性脑病(HIE)新生儿血清白介素(IL)-1β、IL-10、肿瘤坏死因子-α(TNF-α)水平及外周血T细胞亚群的变化。方法选择2006年5月至2009年10月本院新生儿病房收住院的30例HIE患儿为病例组,同期无窒息的足月新生儿30例为对照组,应用ELISA法检测两组新生儿血清IL-1β、IL-10、TNF-α水平,分离外周血单个核细胞,用免疫组化法检测CD3+、CD4+、CD8+、CD4+/CD8+细胞百分率。结果病例组血清IL-1β、IL-10、TNF-α水平(pg/ml)均高于对照组[(16.2±4.6)比(13.0±4.1),(21.6±5.1)比(16.4±4.2),(19.5±0.3)比(12.1±0.3),P均<0.05],CD3+、CD4+、CD8+细胞数量(%)低于对照组[(41.3±7.1)比(45.2±7.3),(36.1±6.6)比(40.0±5.8),(22.9±4.2)比(25.8±4.4),P均<0.05]。结论 HIE患儿存在一定程度的免疫功能紊乱,IL-1β、IL-10、TNF-α和T细胞亚群可能参与了HIE的发病过程。
Objective To investigate the changes of serum interleukin-1β, interleukin-10 (IL-10), tumor necrosis factor-α (TNF-α) and the level of T lymphocyte subsets in neonates with hypoxic-ischemic encephalopathy (HIE) Methods From May 2006 to October 2009, 30 HIE infants hospitalized in neonatal ward in our hospital were selected as the case group. Thirty newborn infants with asphyxia during the same period were selected as the control group. The infants’ Serum levels of IL-1β, IL-10 and TNF-α were detected. Peripheral blood mononuclear cells were isolated and the percentages of CD3 +, CD4 +, CD8 + and CD4 + / CD8 + cells were detected by immunohistochemistry. Results The levels of serum IL-1β, IL-10 and TNF-α in the cases were significantly higher than those in the control group [(16.2 ± 4.6) vs (13.0 ± 4.1), (21.6 ± 5.1) vs (16.4 ± 4.2) , (19.5 ± 0.3) vs (12.1 ± 0.3), P <0.05]. The number of CD3 +, CD4 + and CD8 + cells in the control group was significantly lower than that in the control group [(41.3 ± 7.1) vs (45.2 ± 7.3) and (36.1 ± 6.6) ) (40.0 ± 5.8), (22.9 ± 4.2), (25.8 ± 4.4), P <0.05 respectively. Conclusion There is a certain degree of immune dysfunction in children with HIE. IL-1β, IL-10, TNF-α and T cell subsets may be involved in the pathogenesis of HIE.