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非甲非乙型肝炎的病原至今未能确定,其诊断主要是排除甲型和乙型肝炎。本研究用间接免疫荧光方法(IF)对已被接种证明可传染给正常人而引起非甲非乙型肝炎的病人血清注射给10只黑猩猩,造成试验性感染模型,每周采取肝活检组织进行IF检查。试验方法阳性底片为经IF检查证明肝细胞核抗原阳性的非甲非乙型肝炎黑猩猩的肝活检切片。阴性底片为未感染的或HBsAg阳性或急性甲型肝炎的黑猩猩的肝活检切片。阳性血清取自经IF法检查确有对核抗原阳性抗体的黑猩猩的血清。
The pathogens of non-A, non-B hepatitis have not been confirmed so far, and their diagnosis mainly excludes A and B hepatitis. In this study, ten chimpanzees were injected with sera from patients who had been vaccinated to prove that they could be transmitted to normal people and caused non-A, non-B hepatitis by indirect immunofluorescence assay (IF), resulting in a model of experimental infection. Weekly liver biopsy was performed IF check. Test Methods Positive negatives were liver biopsies from non-A, non-B hepatitis chimpanzees who were positive for hepatocyte nuclear antigen by IF examination. Negative negatives were liver biopsies from chimpanzees from uninfected or HBsAg-positive or acute Hepatitis A patients. Positive sera were taken from sera from chimpanzees that had positive nuclear antigen-positive antibodies by the IF method.