Genome-wide identification, expression analysis of auxin-responsive GH_3 family genes in maize(Zea m

来源 :Journal of Integrative Plant Biology | 被引量 : 0次 | 上传用户:lzflivecn
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Auxin is involved in different aspects of plant growth and development by regulating the expression of auxinresponsive family genes. As one of the three major auxinresponsive families, GH3(Gretchen Hagen3) genes participate in auxin homeostasis by catalyzing auxin conjugation and bounding free indole-3-acetic acid(IAA) to amino acids.However, how GH3 genes function in responses to abiotic stresses and various hormones in maize is largely unknown.Here, the latest updated maize(Zea mays L.) reference genome sequence was used to characterize and analyze the Zm GH3 family genes from maize. The results showed that 13 Zm GH3 genes were mapped on fi ve maize chromosomes(total10 chromosomes). Highly diversi fi ed gene structures and tissue-speci fi c expression patterns suggested the possibility of function diversi fi cation for these genes in response to environmental stresses and hormone stimuli. The expression patterns of Zm GH3 genes are responsive to several abiotic stresses(salt, drought and cadmium) and major stress-relatedhormones(abscisic acid, salicylic acid and jasmonic acid)Various environmental factors suppress auxin free IAA contents in maize roots suggesting that these abiotic stresses and hormones might alter GH3-mediated auxin levels. The respon siveness of Zm GH3 genes to a wide range of abiotic stresses and stress-related hormones suggested that Zm GH3 s are involved in maize tolerance to environmental stresses. A one of the three major auxin responsive families, GH3 (Gretchen Hagen 3) genes participate in auxin homeostasis by catalyzing auxin conjugation and bounding free indole-3- acetic acid (IAA) to amino acids. How, GH3 genes function in responses to abiotic stresses and various hormones in maize is substantially unknown. Here, the latest updated maize (Zea mays L.) reference genome sequence was used to characterize and analyze the Zym GH3 family genes from maize. The results showed that 13 Zm GH3 genes were mapped on fi ve maize chromosomes (total 10 chromosomes). Highly diversi fi ed gene structures and tissue-speci fi c expression patterns suggested the possibility of function diversi fi cation for these genes in response to environmental stresses and hormone stimuli. The expression patterns of Zm GH3 genes are responsive to several abiotic stresses and cadmium) and major stress-related hormones (abscisic acid, salicylic acid and jasmonic acid) IAA contents in maize roots suggesting that these abiotic stresses and hormones might alter GH3-mediated auxin levels. The responsivity of Zm GH3 genes to a wide range of abiotic stresses and stress-related hormones suggested that Zm GH3 s are involved in maize tolerance to environmental stresses.
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