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探讨不同剂量组的补阳还五汤益气组与活血组有效成分在MCAO大鼠体内的药动学特征.复制大鼠脑缺血再灌注动物模型,建立补阳还五汤微透析样品的液质联用检测方法,研究补阳还五汤益气组低、中、高剂(3.09,6.17,12.34 g/Kg),活血组注射液给药剂量为2.32 g/Kg,股静脉给药后脑缺血再灌注损失大鼠中有效成分的药动学特征.补阳还五汤不同给药组中芒柄花素和芍药苷药动学过程符合一房室模型模型,低剂量益气组和活血组中芒柄花素和芍药苷的t1/2分别为(88.43±3.82,69.18±0.11)min,MRT分别为(138.56±4.83,113.62±2.42) min,AUC0-t分别为(28 488.35±4800.32,140 614.80±23 954.05) ng/mL·min;中剂量益气组和活血组中芒柄花素和芍药苷的t1/2分别为(82.16±1.78,67.08±3.69) min,MRT分别为(127.95±2.70,116.58±4.13) min,AUC0-t (48 619.25±6745.75,159 026.00±15 003.33) ng/mL·min;高剂量益气组和活血组中芒柄花素和芍药苷的t1/2分别为(80.29±1.12,69.69±0.87) min,MRT分别为(128.79±1.46,118.78±4.56) min,AUC0-t分别为(109 942.90±13 101.83,189 417.90±22 311.00) ng/mL·min.高剂量益气组能延长芍药苷t1/2、MRT,能延缓芍药苷在脑内被代谢,有利于芍药苷在脑内维持更持久的作用时间.“,”To investigate the pharmacokinetics of effective components of Bu Yang Huang Wu Decoction (BYHWD) with different dosages of Astragalus (Yiqi groups and Huoxue groups) applicating in rats with middle cerebral artery occlusion (MCAO).Replicating the animal model of cerebral ischemia-reperfusion in rats,establishing the liquid-mass spectrometry method for the determination of BYHWD and researching the pharmacokinetics of effective components of yiqi groups of BYHWD with different dosages of astragalus (3.09,6.17,12.34 g/Kg) and Huoxue goups (2.32 g/Kg) when applicated seperately in the rats suffering from cerebral ischemia reperfusion injury after femoral vein administration.The pharmacokinetics of formononetin and paeoniflorin in the different dosage groups of BYHWD met the one-compartment model,and the t1/2 of formononetin and paeoniflorin in the low-dose Yiqi and Huoxue groups were (88.43±3.82,69.18±0.11) min,MRT were (138.56±4.83,113.62±2.42) min,and A UC0-t were (28 488.35±4800.32,140 614.80±23 954.05) ng/mL.min;The t1/2 of formononetin and paeoniflorin in the middle-dose Yiqi group and Huoxue group were (82.16±1.78,67.08±3.69) min,and MRT were (127.95±2.70,116.58±4.13),AUC-t were (48 619.25±6745.75,159 026.00±15 003.33) ng/mL·min;The t1/2 of formononetin and paeoniflorin in the high-dose Yiqi and Huoxue groups were (80.29±1.12,69.69±0.87) min,and MRT was (128.79±1.46,118.78±4.56) min,AUC0-t were (109 942.90±13 101.83,189 417.90±22 311.00) ng/mL·min.The concentration rate of formononetin t1/2 brain was decreased with increase of Astragalus dose.However,no significant difference between these two variables was found during experiments.Furthermore,the experiments showed that the increasing dose of astragalus would affect the pharmacokinetic behavior of paeoniflorin in the Huoxue groups.More specifically,the result showed that paeoniflorin can be metabolized more slowly in the body when applicated in high dose of the jaundice administration groups.In this way,the effect of paeoniflorin can be lasted for longer time in the body and brain.