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目的:利用生物信息学方法分析人Endophilin B1基因以及蛋白的结构,为进一步研究其功能和参与的调控机制提供一定的理论依据。方法:通过GenBank搜索Endophilin B1基因及蛋白序列,采用生物信息学方法分析该基因在不同物种中的差异,分析该蛋白的亚细胞定位,二级结构,功能域以及抗原表位。结果:该基因编码一个长度为365个氨基酸的蛋白,具有两个BAR和SH3两个功能域。Endophilin B1蛋白理论分子量为40796.3,理论等电点为5.78。二级结构中α螺旋(H)占56.44%,β折叠(E)占5.48%,无规卷曲占38.08%。Endophilin B1蛋白含有4个可能的N连接糖基化位点,5个潜在的酪蛋白激酶II磷酸化位点,7个豆蔻酰基化位点,3个PKC磷酸化位点以及2个酪氨酸激酶磷酸化位点。并进一步利用DNAstar软件分析了了该蛋白的抗原表位。结论:利用生物信息学预测出的结构和功能信息,能为Endophin B1蛋白的相关研究提供一定的信息基础。
OBJECTIVE: To analyze the gene and protein structure of human Endophilin B1 by using bioinformatics methods, and to provide some theoretical basis for further study on its regulatory mechanism of function and participation. Methods: The gene and protein sequence of Endophilin B1 were searched by GenBank. The difference of the gene in different species was analyzed by bioinformatics method. The subcellular localization, secondary structure, functional domain and antigen epitope of the protein were analyzed. Results: This gene encodes a 365 amino acid protein with two BAR and SH3 domains. The theoretical molecular weight of Endophilin B1 protein is 40796.3 and the theoretical isoelectric point is 5.78. In the secondary structure, α-helix (H) accounted for 56.44%, β-sheet (E) accounted for 5.48% and random coil 38.08%. The Endophilin B1 protein contains 4 possible N-linked glycosylation sites, 5 potential casein kinase II phosphorylation sites, 7 myristoylation sites, 3 PKC phosphorylation sites and 2 tyrosines Kinase phosphorylation site. The DNA epitope was further analyzed by DNAstar software. Conclusion: The structural and functional information predicted by bioinformatics can provide some information basis for the related research of Endophin B1 protein.