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显微分光光度法是利用分光光度法的原理,以一定波长的单色光在显微镜下对生物样品微细结构中的化学物质进行定量测定。显微分光光度法测定的不是一个细胞,而是一个细胞群体内的某种物质(如DNA),其中各个细胞内的物质含量不完全相同,然而,一个细胞群体的某物质含量有一定的分布,通常用组织图来表示,正常细胞胞核的DNA含量只与染色体的数目有关,如呈规律性的成倍增加,即为2倍体、4倍体等。用这种方法,可以鉴定正常细胞受到某种外界环境刺激后所发生的变化,如癌变等。我们采用这种技术探测大白鼠受Co~(60)γ射线照射后外周血淋巴细胞核内DNA含量的变化。一、材料和方法选择体重为150g—200g的雄性大白鼠,每16—20只为一批,进行Co~(60)r照射,剂量
Micro-spectrophotometry is the use of spectrophotometric principle, to a certain wavelength of monochromatic light microscopy of biological samples in the fine structure of the chemical quantitative determination. Microscopic spectrophotometry is not a cell, but a cell population of a substance (such as DNA), in which the content of each cell is not exactly the same, however, a cell population of a substance content distribution , Usually histogram to indicate that the DNA content of normal cell nuclei only with the number of chromosomes, such as the regular doubled, that is, 2-fold, 4-fold and so on. In this way, you can identify the normal cells by some kind of external environment stimulated changes, such as cancer and so on. We used this technique to detect changes in the DNA content of peripheral blood lymphocytes in rats exposed to Co ~ (60) γ-rays. First, materials and methods Male rats weighing 150g-200g were selected, and the rats were treated with Co ~ (60) r for every 16-20 days. The dose