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目的：为探讨糖尿病的发病机理，观察糖尿病小鼠心、脑、脾、肾、胰腺的ＧＳＨ－Ｐｘ、ＳＯＤ、Ｈ２Ｏ２ａｓｅ、ＭＤＡ及ＮＯ含量的变化及硒、大蒜素单用与合用对糖尿病小鼠抗氧化功能的影响。结果：糖尿病小鼠心、脾、肾、胰腺ＧＳＨ－Ｐｘ、ＳＯＤ、Ｈ２Ｏ２ａｓｅ活性均呈下降或呈下降趋势，ＭＤＡ含量升高，其中脾和胰腺的变化较为显著，除心脏ＮＯ含量降低外，脾、肾、胰腺含量明显升高，对脑无显著影响。硒、大蒜素对不同抗氧化酶活性影响各不相同，硒主要提高ＧＳＨ－Ｐｘ及Ｈ２Ｏ２ａｓｅ活性，而大蒜素对ＳＯＤ酶活性影响较大，但它们均能降低脾、肾、胰腺ＭＤＡ和ＮＯ含量。结论：糖尿病的主要病变部位在脾、肾、胰腺，硒、大蒜素能改善其抗氧化功能。
OBJECTIVE: To investigate the pathogenesis of diabetes mellitus, the changes of GSH-Px, SOD, H2O2ase, MDA and NO in heart, brain, spleen, kidney and pancreas of diabetic mice were observed and the effects of selenium and allicin alone and in combination on diabetic mice Antioxidant effects. Results: The activities of GSH-Px, SOD and H2O2 in heart, spleen, kidney and pancreas of diabetic mice all decreased or descended, the content of MDA increased, and the changes of spleen and pancreas were significant. , Kidney, pancreas increased significantly, no significant effect on the brain. Selenium and allicin had different effects on the activity of different antioxidant enzymes. Selenium mainly increased the activities of GSH-Px and H2O2ase, while allicin had a significant effect on the activity of SOD, but all of them reduced the contents of MDA and NO in the spleen, kidney and pancreas . Conclusion: The main lesion sites of diabetes are spleen, kidney, pancreas, selenium and allicin, which can improve their anti-oxidant function.