论文部分内容阅读
为探讨TaUGT73D1基因在小麦花发育过程中雄蕊和雌蕊形成的作用,本研究利用小麦三雌蕊突变体TP和雄蕊同源转化型不育突变体HTS-1两个品种,从中克隆得到了TaUGT73D1(UDP-glycosyltransferase73D1)的3个同源基因TaUGT73D1-2A、TaUGT73D1-2B、TaUGT73D1-2D,通过基因碱基序列分析表明TaUGT73D1-2A、TaUGT73D1-2B、TaUGT73D1-2D基因序列全长分别为1 907 bp、1 912 bp、1 905 bp,开放阅读框分别为1 365 bp、1 467 bp、1 461 bp,分别编码454、488、486个氨基酸残基,通过与小麦基因组比对,发现这3个同源基因分别位于染色体2AL、2BL、2DL上。TaUGT73D1-2A、TaUGT73D1-2B、TaUGT73D1-2D与乌拉尔图小麦Tu UGT73D1的编码区相似度分别为97.68%、96.52%、99.05%,蛋白序列相似度分别为73.57%、95.08%、95.70%。系统进化分析表明,该类基因与单子叶植物的UGT基因聚在一起,表明其亲缘关系较近,而与双子叶植物相隔较远,表明其亲缘关系较远。利用Real-time PCR分析TTaUGT73D1-2A、TaUGT73D1-2B、TaUGT73D1-2D基因在不同花器官雌蕊(P)、雄蕊(S)及雌蕊化雄蕊(PS)中表达模式。结果表明,该类基因在雄蕊中表达量最高,在雌蕊及雌蕊化中几乎不表达。因此推测TaUGT73D1基因可能与小麦雄蕊同源转化为雌蕊性状有关。本研究为进一步探讨小麦TaUGT73D1基因的功能提供了理论依据。
In order to investigate the role of TaUGT73D1 in the formation of stamens and pistils during the development of wheat flower, we studied the effects of TaUGT73D1 on the formation of stamens and pistils. -Galcosyltransferase73D1). The full-length TaUGT73D1-2A, TaUGT73D1-2B and TaUGT73D1-2D gene sequences were 1 907 bp and 1 respectively by gene base sequence analysis (TaUGT73D1-2A, TaUGT73D1-2B, TaUGT73D1-2D) 912 bp and 1 905 bp respectively. The open reading frames (ORFs) were 1 365 bp, 1 467 bp and 1 461 bp, respectively encoding 454,488,486 amino acid residues. By comparing with the wheat genome, Located on chromosome 2AL, 2BL, 2DL respectively. The similarities of the coding regions of TaUGT73D1-2A, TaUGT73D1-2B and TaUGT73D1-2D with Tu UGT73D1 were 97.68%, 96.52% and 99.05%, respectively. The similarity of the protein sequences were 73.57%, 95.08% and 95.70%, respectively. Phylogenetic analysis showed that these genes clustered with monocotyledonous UGT genes, indicating that they are closely related to each other and distant from dicotyledons, indicating that they are distantly related. The expression patterns of TTaUGT73D1-2A, TaUGT73D1-2B and TaUGT73D1-2D genes in pistil (P), stamen (S) and pistillate stamens (PS) of different floral organs were analyzed by Real-time PCR. The results showed that the highest expression level of these genes in stamens, almost no expression in pistil and pistil. Therefore, it is speculated that the TaUGT73D1 gene may be related to the homologous transformation of wheat stamen to the pistil character. This study provides a theoretical basis for further exploring the function of TaUGT73D1 gene in wheat.