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背景:如何减轻甚至消除脂多糖(LPS)引起的全身炎症反应是目前研究的热点;库普佛细胞(KC)的激活与LPS的这种作用密切相关,但关于其具体的机制尚有待于进一步研究。目的:探讨LPS对KCCD14表达的影响及CD14在LPS激活KC中的意义。设计:完全随机前后对照研究。地点和对象:在第三军医大学西南医院病理学研究所完成。大鼠KC来源于Wistar大鼠,购自第三军医大学实验动物中心。干预:在分离培养大鼠KC的基础上,作者应用LPS直接或LPS刺激KC后产生的介质刺激新培养的KC,或在血清存在的情况下加入抗CD14单抗或在无血清的情况下单独加入LPS等措施刺激KC细胞。主要观察指标:各种条件下CD14mRNA的表达及其蛋白合成的变化、培养KC中上清中肿瘤坏死因子α(TNF-α)、白细胞介素6(IL-6)和一氧化氮浓度。结果:①CD14mRNA表达及其蛋白合成在正常KC中极弱,分别为0.035和19.91±2.89,浓度为10mg/L的LPS分别为0.73和676.79±24.95,其量与LPS浓度呈剂量依赖性相关。浓度为10μg/L的LPS刺激后KC中CD14mRNA表达及其蛋白合成在30min即明显增强,分别为0.56和548.86±40.43,至6h达高峰(0.54和673.91±35.08)。②在血清存在时加入抗CD14单抗或在无血清时单独加入LPS,可明显降低KCTNF-α,IL-6和一氧化氮的释放。而后者如果同时加入LBP,则可?
BACKGROUND: How to reduce or even eliminate the systemic inflammatory response induced by lipopolysaccharide (LPS) is the current research hotspot. The activation of Kupffer cells (KC) is closely related to the effect of LPS, but its specific mechanism needs to be further studied the study. Objective: To investigate the effect of LPS on the expression of KCCD14 and the significance of CD14 in LPS-activated KC. Design: A completely randomized anteroposterior study. Location and Subject: Completed at the Institute of Pathology, Southwest Hospital, Third Military Medical University. Rats KC from Wistar rats were purchased from the Third Military Medical University Experimental Animal Center. Intervention: On the basis of isolating and culturing rat KCs, the authors stimulated newly cultured KCs with LPS-stimulated medium produced by LPS stimulation or LPS alone, or anti-CD14 mAbs in the presence of serum or in the absence of serum Join LPS and other measures to stimulate KC cells. MAIN OUTCOME MEASURES: Expression of CD14 mRNA and its protein synthesis under various conditions, TNF-α, IL-6 and NO concentration in the culture supernatant of KC. Results: ①CD14 mRNA expression and its protein synthesis were very weak in normal KC, which were 0.035 and 19.91 ± 2.89, respectively. The LPS at the concentration of 10 mg / L was 0.73 and 676.79 ± 24.95, respectively, which was correlated with the LPS concentration in a dose-dependent manner. CD14mRNA expression and protein synthesis in KC stimulated with LPS at a concentration of 10μg / L for 30min significantly increased at 0.56 and 548.86 ± 40.43, reaching the peak at 6h (0.54 and 673.91 ± 35.08), respectively. ② The addition of anti-CD14 mAb in the presence of serum or the addition of LPS alone without serum can significantly reduce the release of KCTNF-α, IL-6 and nitric oxide. The latter if you join the LBP, can?